RAPD Analysis Of Genetic Relationships Among The Cultivated Eggplant(Solanum Melongena L.) And Wild Relatives (Solanaceae)

Eggplant(Solanum melongena) originates from Indian sub-continent and Asian tropics, China is one of secondary centers of eggplant origin. Our objective was to study the genetic diversity of 16 eggplant materials. The results as following:1. The optimal PAPD-PCR system in watermelon was established. Amplification was performed in 10μL reaction mixture containing 1×PCR buffer, 3mM MgCl2 , 200μM of each dNTP, 0.2μM primer, 0.5 unit Taq polymerase and approximately 25 ng DNA. The samples were run in a ASTEC PC-808 thermocycler programmed for a cycle of 3 min at 94℃, 45 cycles of 30 sec at 94℃, 30 sec at 39℃and 1 min at 72℃. These cycles were followed by 7 min at 72℃for extra elongation.2. The 57 primers amplified 551 fragments in the 16 accessions of S. melongena and its relatives, in which 274 fragments were polymorphic. The number of fragments per primer varied from 3 to 16, with a mean of 9.67.3. Data were analysed with computer software NTSYSpc (version 2.10). The RAPD-based genetic similarity ranged from 0.35 to 1.00. UPGMA cluster analysis showed that the tested materials could be divided into three groups at the level of genetic similarity coefficient 0.83.