| Two-phase experiments were conducted to study the effect of dietary lysine,methionine and threonine on growth performances,blood biochemical parameters,apparent metabolism rates,slaughter performance and meat quality of Hepu goose.The orthogonal 3×3 factorial trial was used and 9 kinds of diet were designed to determine the proper requirements of dietary lysine,methionine and threonine of Hepu green goose(0-4W)and Hepu gosling(5-8W)In experiment phase one,270 one day old Hepu green geese were allotted to 9 dietary treatments.Each treatment of 30 geese had 3 replicates. The experimental diets were designed to contain 0.85%,1.00%and 1.15% lysine;0.35%,0.45%and 0.55%methionine;0.55%,0.65%and 0.75% threonine,respectively.The aim was to study the effect of dietary different lysine,methionine and threonine levels on growth performance and blood biochemical parameters,also to determinate appropriate levels of the three nutrients in Hepu green goose.The results showed that:(1)Lysine factor had significantly effect on average daily gain(P＜0.05),and significantly effect on feed to gain ratio (P＜0.05),The highest average daily gain was observed in 1.00%lysine treatment group;The average daily gain was significantly higher in 0.45% methionine level than in the other levels(P＜0.05);No significant difference on growth performances was obtained in dietary threonine treatments (P＞0.05).(2)The concentration of glyceride in serum was lower in 1.00%lysine treatment than in the others(P＜0.05);Methionine factor had significantly effect on the concentration of uric acid in serum(P＜0.05)and the lowest concentration of uric acid was observed in green goose fed 0.55% lysine level diet;The lowest urea acid in serum was obtained by 0.75%of dietary threonine level(P＜0.05)Comparing all quotas,under the condition of this experiment,the optimum lysine,methionine,threonine level for 0-4 weeks of Hepu green goose can be:1.00%,0.45%,0.55%,respectively.In experiment phase two,216 twenty-nine days old Hepu goslings were allotted to 9 dietary treatments.Each treatment of 24 geese had 3 replicates. The experimental diets were designed to contain 0.78%,0.88%and 0.98% lysine;0.30%,0.40%,and 0.50%methionine;0.44%,0.54%and 0.64% threonine,respectively.The aim was to study the effect of dietary different lysine,methionine and threonine levels on growth performance and relative parameters,also to determinate appropriate levels of the three nutrients in Hepu gosling.The results showed that:(1)The factor of lysine had significantly effect on average daily gain and average daily feed intake(P＜0.01),The highest average daily gain was obtained in 0.88%lysine level diet;The factor of methionine had significantly effect on feed to gain ratio(P＜0.01),The lowest feed to gain ratio was observed in group received 0.40%methionine level diet;There was no significant difference in growth performances among the 3 threonine levels(P＞0.05).(2)Lysine factor had significantly effect on the concentrations of uric acid(P＜0.05)and glyceride(P＜0.01)in serum.The lowest uric acid and glyceride in serum were obtained by 0.98% and 0.88%of dietary lysine level,respectively;The concentration of glyceride was significantly lower in 0.40%methionine level than in the other levels (P＜0.05);There was no significant difference in blood biochemical parameters among the 3 threonine levels(P＞0.05).(3)The apparent metabolism rates of lysine(P＜0.05),methionine(P＜0.01)and threonine (P＜0.01)increased with increasing lysine,methionine,threonine intakes, respectively;threonine factor had significantly effect on apparent metabolism rates of crude protein(P＜0.05).The highest apparent metabolism rates of crude protein was observed in treatment fed 0.44%threonine level diet. Lysine and threonine factors had significantly effect on apparent metabolism rates of arginine(P＜0.05).(4)No significant effect on dressed percentage, percentage of half-eviscerated yield,percentage of eviscerated yield, percentage of leg muscle,percentage of breast muscle and percentage of abdominal fat were observed among the treatment groups(P＜0.05) Howerer,with increasing amino acid intake,those indexes tended to increase expect abdominal fat percentage.(5)Lysine factor had significantly effect on cutting value of leg muscle and meat colour of breast muscle(P＜0.05).The lowest cutting value and the best colour of leg muscle was obtained by 0.78% and 0.88%of dietary lysine level,respectively;Lysine factor had significantly effect on cutting value of breast muscle,percentage of retaining water of breast muscle and leg muscle(P＜0.01).The lowest cutting value of breast muscle,percentage of retaining water of breast muscle and leg muscle were obtained by 0.78%,0.98%and 0.98%of dietary lysine level,respectively. Methionine factor had significantly effect on percentage of retaining water of leg muscle(P＜0.05)and breast muscle(P＜0.01).The lowest percentage of retaining water of leg muscle and breast muscle were obtained by 0.40%of dietary methionine level.Threnine factor had significantly effect on percentage of retaining water of breast muscle and meat colour of leg muscle (P＜0.01).The lowest percentage of retaining water of breast muscle and the best colour of leg muscle were obtained by 0.54%and 0.64%of dietary threnine level,respectively.(6)Lysine factor had significantly effect on the absolute weight of thymus gland and the index of thymus gland(P＜0.01). The higtest absolute weight of thymus gland and index of thymus gland were obtained by 0.98%of dietary lysine level.Methionine factor had significantly effect on the index of thymus gland(P＜0.05).The higtest index of thymus gland was obtained by 0.30%of dietary methionine level.Threnine factor had significantly effect on the absolute weight of gumboro and the index of gumboro(P＜0.05).The higtest absolute weight of gumboro and index of gumboro were obtained by 0.44%of dietary threnine level.Comparing all quotas,under the condition of this experiment,the optimum lysine,methionine,threnine level for 5-8 weeks of Hepu gosling can be:0.88%,0.40%,0.44%,respectively.
|
PDF Full Text Request