| Seeds hardness and seeds vigor were studied on the legume seeds of Astragalus fenzelianus Pet.-Stib.and Vicia amoena Fisch. to select effective treatments to break the hard seeds coat thus improve legume seeds germination;to partially reveal the relationship between hardness of seeds and their vigor;to develop the simple,exact test regulations of vigor test for legume seeds.through physiological index detecting,the effects of low temperature stress on physiological characteristic werw studied to explore the resistance-cold adpation mechanism,provide reference for legume seed quality evalution and legume breeding.Results showed that eroding in 98%(w/v)sulfuric acid for 10 min for the seeds of Astragalus fenzelianus,20 min for Vicia amoena was of most effective to break the hard seeds oat while maintain reasonable germination rate. The vigor of coat-broken hard seeds were higher than that of normal seeds. Taking the germination rate as standard, remarkable correlation were existed between seed vigor and germination index,vitality index,relative electric conductivity of plasma membrane and content of MDA,thus could be effective index to evaluate the vitality of legume seeds.The vigor of hard seeds is the important complement in the evaluation of legume seeds quality;Effects of low temperature stress on physiological characteristic of A.fenzelianus seedlings and V. amoena seedlings were studied. The result showed that the conductance rate,the content of soluble sugar,the content of proline increased ;Effects of low temperature stress on Physiological Characteristic in light and dark treatment were studied. The result showed that the conductance rate and the activity of SOD increased,The content of Chlorophyll decreased,Contracted with low temperature dark treatment,the effects is more significant in low temperature and light treatment. according to the changes of these index,the ability of chill resistance of A.fenzelianus seedlings is higher than that of V. amoena. |
PDF Full Text Request