Study On Extraction And Isolation Of Physiologically Active Substances From Garlic And Its Antibacterial Activity And Its Inhibitive Effect On Melanoma Cell

Our country is garlic's main producer country and the export State, the garlic variety is diverse, the quality of material is fine. But the Western country exports the garlic to our country to implement counter-dumping and the quota limit in recent years, in addition our country garlic process technology's backwardness, has caused the agricultural losses. In order to alleviate in this agricultural production the question, needs to study the technique content to be high, result good garlic intensive processing technology.Established has withdrawn the separation alliin, alliinase and allicin method from the fresh garlic. Alliin by HPLC detection is purity of 98%, compared with the standard garlic. Alliinase vigor unit is 2472U/mg. The sativin content is 172.9g/kg.Through the determination of alliin, alliinase, mixture of alliin and alliinase, allicin and garlic juice to ten kind of pathogenesis bacterium's bacteriostasis function, discovered finally they have the obvious bacteriostasis function to ten kind of pathogenesis bacteria. The initial contact mixture of alliin and alliinase ammonia sour enzyme to supplies to try the fungus the bacteriostasis function to be strong, and MIC is lower than the allicin.Has compared alliin, alliinase, mixture of alliin and alliinase, allicin and garlic juice for the first time to five kind of plant cause of disease fungus's bacteriostasis effect, finally discovered that alliinase, mixture of alliin and alliinase, allicin and garlic juice have the obvious bacteriostasis function to five kind of plant cause of disease fungus. Through MIC, hypha suppression rate and the spore germination's rate discovery quite, the bacteriostasis effect from to weak is in turn: mixture of alliin and alliinase > allicin > garlic juice > garlic ammonia acid.Alliinase, mixture of alliin and alliinase, allicin and garlic juice affect strongly obviously to five kind of plant cause of disease fungus's bacteriostasis function compared to ten kind of pathogenesis bacterium's bacteriostasis.Has studied the allicin and mixture of alliin and alliinase for the first time to the melanoma cell in vitro multiplication and the cell cycle influence, finally indicated that mixture of alliin and alliinase multiplies to melanoma cell in vitro suppresses rate is higher than the allicin, suppression rate achieves 92%. They hinder the melanoma cell cycle in the G0/G1 time.