| Phellinus baumii is one kind of precious for medicinal purposes fungus, its for medicinal value obtained the widespread attention; In tradition, the polysaccharide is extracted from fruiting body of phellinus baumii, but, as a result of its habitat particularity, fruiting body which forms in the nature is scarce, and fruiting body's manual cultivation still was at the start stage, meanwhile, the mycelia in liquid ferment have the raise cycle short and stable product quality that is advantageous for industrialization production, and the mycelia have the remarkable pharmacology activeness, it had been proven by active experiment in vivo and in vitro that the submerged culture mycelia extraction is of cells-mediated immunity, the wide range immune stimulation, the anti-tumor activeness, the blood sugar reducer, liver function protection and so on. The intracellular polysaccharide (IPS) is important active constituent in mycelia, therefore the IPS's yield has decided the mycelial medicinal value; Ferment liquor also includes some active extracellular polysaccharide (EPS) and protein, was confirmed has the pharmacological activeness, therefore, the active components in ferment liquor are a important parameter for medicinal purposes value.This research begins first from the optimization for medium compositions for IPS of P. baumii in submerged culture, and screens out the optimal culture medium combination according to dry mycelial weight (DW) and IPS these two parameters. First, it establishes screening experiments for four kinds of single factor, they are the initial pH, carbon source, nitrogen source, the carbon nitrogen (C/N) ratio, then takes three good levels in each factor to enter the next step orthogonal experiment. According to the orthogonal array design, the relative optimum medium compositions for IPS production are initial pH 5.0, soybean powder, soluble starch, C/N ratio of 1:1, the production obtains 2.70 g/L. According to Intuitive analysis and Variance analysis, the main factor of IPS is initial pH (A), the main factor of mycelial dry weight is nitrogen (B), A and B are independence with each other, correlation coefficient of the results of IPS content and mycelial dry weight was -0.16 (P>0.05), it shows there isn't credibility pertinence between IPS content and mycelial dry weight. This research next begins from the strain selective breeding, the breeding of high-yield strain of IPS is performed by protoplast mutagenesis of strain P. baumii using ultraviolet radiation, then choosing the optimal parameters on production and regeneration of protoplast of P. baumii, the mutant 129~# was abtained, which grows faster than parent strain P. baumii and has raised the DW from 1.95g/L to 2.77g/L and IPS content from 7.83% to 9.95% compared with parent strain P. baumii, the high-yield character is of steady descendiblity; Meanwhile, genetic differences between the mutant 129~# and its parent strain PB are primarily compared through sequence-related amplified polymorphism (SRAP), the results shows that genetic differences are very obviouse between the parent strain and its mutant.This research finally has confirmed initially the pharmacology activeness of IPS and EPS of P. baumii. It has been proved that IPS solution has the obvious inhibitory action to the HCT cell, and assumes concentration dependence; IPS solution solution can activate mouse's immune cell—the abdominal macrophage, which explains IPS has the immunity regulation to organism. Hemoglutination activeness of EPS has been confirmed in this experiment, hemoglutination activeness has assumed the time and the concentration dependence, which explains the hemoglutination active metabolite only is exuberant in certain periods of growth secretion.
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