Immunomodulatory Effect Of Dietary Intake Of Echinacea Purpurea Powder On Performance And Immune System Of Cyprinus Carpio L (Carp)

Cyprinus carpio L(carp) were used for examining the echinacea purpurea extract as a feed additive on their performances effect.Two hundred and fourty carps were randomly individed into 4 groups.In this 4 groups,the one is control,the group 1,group 2 and group 3 are supplied with 0 mg/kg(control) Echinacea purpurea extract,1000mg/kg Echinacea purpurea extract,2000mg/kg Echinaceapurpurea extract and 4000mg/kg Echinacea purpurea extract.At the end of 30-d feeding trial,determine the relative growth rate(RGR),adding-weight,feed coefficient rate(FCR),ingestion apprtite and survival rate.Aider the performance trial examined carp resistance against the infection from Aeromonas hydrophila.To determine the effects of echinacea purpurea extract on the innate immune response of Cyprinus carpio L(carp),fish were fed diets containing 0(control),1000mg/kg,2000mg/kg and 4000mg/kg Echinaceapurpurea extract,for 7,14,21,28 and 35days.Lysozyme,blood leucocyte phagocytica ctivity,serum albumin,serum total protein and serum immunoglobulin (including IgM,IgG and IgA) were studied at each of the assay times.After 40 days,the AKP,ACP,SOD and MDA in tissues including serum,liver,kinder and muscel were detected.The results showed that the survival rate and FCR had no enhancement of every group in performance trial,meanwhile the Lysozyme,blood leucocyte phagocytica ctivity,serum albumin,serum total protein were variation after 21 or 28 days;immunoglobulin IgA had no change in whole trial cycle,the contents of IgM had significant difference(P＜0.01) after 28 and 35 days and IgG of 2000mg/kg group had significant difference in 21 and 35 days.Compared with the control group,the echinacea purpurea extract had a good effect for the AKP,ACP,SOD activitys and MDA contents.The infection trial result showed the echinacea purpurea extract had a good effect on the carp resistance against the infection from Aeromonas hydrophila.The trial results indicated echinacea purpurea extract could enhanced the growth performance and anti-diseases effect.It is a superordinary additive on aquatic.For the immunomodulatory efect of echinaceapurpurea extract and take the economy and efficiency into account,it is suggested that the feasible dosage was about 2000mg/kg.