Effects Of LXRα Agonist On TG Accumulation And MRNA Expression Of Lipid Metabolism Genes In Goose Hepatocytes

LXR (Liver x-activate receptors) is an important lipid sensor. By regulating the synthesis of fatty acid, LXR controls the balance of lipid metabolism, which is implemented by regulating transcription of key gene (SREBP-1, LPL, FAS and LXRα). In this study primary hepatocytes of Sichuan white Goose and Landes Goose were used as research materialst to study the effects of LXRαagonist on expression of TG accumulation, FAS activity and mRNA expression of LXRα, SREBP-1, FAS and LPL. The main results showed as follows:1. With LXRαagonist concentration increasing, the TG contents of Sichuan White Goose and Langde Goose hepatocytes had a trend to increasing. TG accumulation induced by LXRαagonist in hepatocytes of two breeds was max (1.22 mmol/L and 1.67 mmol/L, respectivly). TG accumulation in Langde Goose hepatocytes induced by the same dose of LXRαagonist was higher than that in Sichuan White Goose.2. With the extension of culture time, TG accumulation in hepatocytes of two breeds had a trend to increase when the culture of time was less than 48 hours. However, it reduced at the time of 72 hours. At the same period of culture time, TG accumulation of Langde Goose hepatocytes was higher than that in Sichuan White Goose.3. With LXRαagonist concentration increasing, FAS activities in hepatocytes of two breeds both increased. In the same dose of LXRαagonist, FAS activity in hepatocytes of Langde Goose was higher than that in Sichuan White Goose.4. With the extension of culture time, FAS activities of hepatocytes in both breeds had a trend to increase when the culture of time was less than 48 hours. However, it reduced after 48 hours. The maximum was at the time of 48 hours (231.97 U/ml and 297.56 U/ml, respectivly). At the same period of culture time, FAS activity in hepatocytes of Langde Goose was higher than that in Sichuan White Goose.5. With LXRαagonist concentration increasing, the mRNA expressions of LXRα,SREBP-1,FAS and LPL genes in hepatocytes of Sichuan White Goose and Langde Goose had a trend to increasing. When the dose of LXRαagonist was 10μmol/L , the mRNA expression of above genes reached max. In the same dose of LXRαagonist, the mRNA expression of LXRα,SREBP-1,FAS and LPL genes in hepatocytes of Langde Goose were all higher than these in Sichuan White Goose.6. With the extension of culture time, the mRNA expressions of LXRα,SREBP-1 and FAS genes in hepatocytes of Sichuan White Goose and Langde Goose had a trend to increasing when the culture of time was less than 48 hours. However, it reduced after 48 hours. The maximum was at the time of 48 hours. At the same period of culture, the mRNA expressions of LXRα,SREBP-1 and FAS genes in hepatocytes of Langde Goose were higher than these in Sichuan White Goose.7. The correlation analysis showed that the dose of LXRαagonist had a significant correlation with the FAS mRNA expression in Sichuan White Goose hepatocytes, and it did not have significant correlation with other items which were determined in this experiment and these of Langde Goose hepatocytes, too. TG content, FAS activity induced by LXRαagonist in hepatocytes of both geese had significant correlation with mRNA expressions of FAS, SREBP-1 and LXRαgenes.