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The Primary Research Of Biological Activity Of Bacillus Licheniformis C And L-3

Posted on:2009-10-29Degree:MasterType:Thesis
Country:ChinaCandidate:J TangFull Text:PDF
GTID:2143360248451642Subject:Pesticides
Abstract/Summary:PDF Full Text Request
According to the morphological,physiological and biochemical features, bacterium C and L-3 are identified as Bacillus Iicheniformis.The two strains can control 11 plant diseases which caused funguns,such as Fusarium graminearum,Bipolaris maydis and Sclerotinia sclerotiorum,by mensurating the antimicrobial activity of zymotic fluid,but there is no obvious activty to the 6 bacterium in the test.Trough the determination of biological activity,supernatant and thalli of two strains restrain Bipolaris maydis which is as a direction.It shows that antimicrobial substance is prouduced at fermenting,and the substance is confirmed as a protein primarily,because it can be deposited by 60%(NH4)2 SO4.Determining the hormesis of zymotic fluid of the two stains to three plant seeds, which indicate activity substance in supernatant to stimulate plant growth,and the effect of wheat and corn is much better than that of cabbage.The results show that the substance is not component of culture medium,but the metabolite excreted by the stains. Trough synthesizing Amaranthin by yellow seedling of Amaranthus Gandatus L.,it may be a Cytomin.According to potted plant of wheat and field trial of corn with different treatments, the result of spraying zymotic fluid over leaves and irrigating it in root synchronously demonstrated that the output of wheat and corn can be improved.
Keywords/Search Tags:Bacillus licheniformis, Antimicrobial activity, Antimicrobial protein, Stimulating plant growth., Cytomin
PDF Full Text Request
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