Fowlpox Virus Isolated From The Areas Identified And The Study Of Pathogenicity

Fowlpox poultry industry is harmful to the development of a major infectious diseases, the incidence after a chicken does not normally cause death, chicken mortality rate higher, ranging from 5% to 30%, mucous membrane-type fowlpox more serious symptoms, and sometimes can cause higher Mortality. Fowlpox can diseased growth retardation, egg drop, when the hatching rate lower incidence Zhongqin, if complicated by other infectious diseases, parasitic diseases and health conditions, poor nutrition also can cause a large number of deaths, the mortality rate is as high as 40 percent to 50%.At present, countries in the world for the prevention of mainly Fowlpox vaccination or pigeon fowlpox pox virus live attenuated vaccine, to a large extent reduce the incidence of the fowlpox. But in recent years, fowlpox have increased the incidence of the trend, and the symptoms are severe than in the past, immune failure is also very common. To investigate whether there had been a fowlpox virus mutation, we should establish than the traditional serological methods more sensitive and specific detection method in molecular biology, we have fowlpox virus molecular genetics analysis and PCR method of diagnosis.The clinical trial of the suspected fowlpox with chicken, a virus separation, purification and identification, sequence analysis, the establishment of the fowlpox virus PCR testing methods, and conducted a preliminary application.The first trial of five cases of suspected skin type fowlpox a sampling of diseased chickens and the virus isolation and identification. 10-day-old chicken embryo using urine capsule three times for passage, the virus isolation, the effect of diseases caused by viruses (CPE), agar-proliferation response to prove that the virus was isolated by the fowlpox virus, named FPVSD1~5. According to published fowlpox virus hemagglutinin of the C-type gene sequence, to design primer, FPVSD1~5 isolates were amplified by PCR to the expected 647 bp fragment, after sequencing, comparative analysis of the sequence of the gene sequence variation Research shows that: the sequence and has been published by the fowlpox gene sequences as high as 100%, there was no genetic mutation.According to fowlpox virus gene sequence, design another one pair of primers, amplified by PCR to the expected 704 bp fragment, after sequencing, comparative analysis of the sequence of the gene sequence variation studies show that: the sequence and has been published by the fowlpox Gene sequences as high as 100 % genetic mutation has not occurred. Once again proves that fowlpox virus genome conservative higher.Separation of local use of fowlpox virus on behalf of SPF chicks and eggs of chickens goods to the infection experiments, results showed that: The chicken is not a serious fowlpox symptoms and pathological changes.The establishment of the PCR method, and can be used in molecular biology fowlpox virus diagnosis.