Identification And SSR Markers Linked To Dwarf Gene In Germplasm Line Shannong31504-1

Dwarf germplasm line Shannong31504-1 was isolated by using morphological markers,cytological markers and biochemical markers. Meanwhile,in order to find the molecular marker of the dwarf gene,SSR markers were used.The results are showed just as follows.The dwarf germplasm line Shannong31504-1 was compared with seven main dwarf sources in field characters.The results indicate that the dwarf germplasm line Shannong31504-1 have good field characters and no premature senility.It is very valuable in the area of plant breeding and genetics.Crosses between the dwarf translocation line shannong31504-1 and six high material(ChineseSpring,yanyou361,Et98529,lu95519,zimaiNO.12, zhongyou9501) were made respectively. Plant height of F1 generation near to value of medium parents. Shannong31504-1 / Chinese Spring, Shannong31504-1/ yanyou361 F2 segregation population and Shannong31504-1/ yanyou361// yanyou361 BC1 were carried out. The results are showed that the proportion of the dwarf semi-dwarf and high individuals in the F2 segregating population was 1:2:1,the proportion of the dwarf and high individuals in the BC1population was 1:1.Dwarf gene shows GA insensitivity through GA reaction identification. Cross between dwarf germplasm line Shannong31504-1 and Chinese Spring was carried out for constrncting F2 segregation population. By SSR analysis with 179 SSR and EST-SSR primer pairs located on 2A chromesome in 197 individuals of F2 segregation population and their parents,and Bulked segregating analysis (BSA), we screened two SSR markers(Xwmc522,Xwmc198) that linked to the dwarf gene in Shannong31504-1.The dwarf gene was located on chromorome 2AS and shown to be closely linked to the marker Xwmc522 and Xwmc198 at a genetic disrance of 5.4 cM and 3.2cM respectively. They can be used in MAS directly.