| Canola is an important oilseed crop in the world. Stem rot is the most devastating disease in Brassica.napus and causes yield loss and quality decline, uaually it infectes the plants in the age of later growth period. No cultivar with high resistance against Sclerotinia sclerotiorum has been developed in B. napus, resulting from no resource resistance gene in B.napus. Therefore, limited germplasm of B. napus could be used on breeding to enhance the resistance against S. sclerotiorum. Along with the development of biotechnology, the method of transformation could be used on the resistance breeding of B. napus.Chitinase encoding gene CH5B and oxalate oxidase encoding gene HvOxOh were used in the research. Three vectors including SAG12 : CH5B, SAG12 : HvOxOh and 35S: CH5B were used in B. napus Westar10 transformation. The results showed that 4d seedling was most suitable for transformation and co-cultivation time with Agrobacteriun for 48 h was better. The medium with 4 mg/L 6-BAP was not benefit to explants regeneration and 500 mg/L Carbenicillin reduced plants growth.Thirty two transgenic plants were obtained, thirteen, five, and fourteen of which were transformed with the vector of SAG12 : CH5B, SAG12 : HvOxOh and 35S : CH5B, respectively. Southern blot analysis evaluated the insertion of transgenes in transgenic plants and the expression of transgenes were further confirmed by Northern blot and RT-PCR. The activity of chitinase and oxalate oxidase was increased in senescening leaves of transgenic plants. The inoculation with mycelium was conducted in the detached leaves from transgenic plants to evaluate the resistance against S. sclerotiorum. No enhancement of resistance in transgenic plants was detected.
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