Study Of Influence On Germination And Seedlings Of Different Winter Wheat Cultivars Under Salinity Stress And Alleviatory Effect Of Ca Nutrition

The experiment was conducted in laboratory and greenhouse of Agriculture Science Department of De Zhou University.Two different cultivars of winter wheat(DK961,high salt-tolerant;JN17) were tested. DK961 was a salt tolerant winter wheat cultivar and JN17 was a popular winter wheat variety demonstrated in Shandong Province.In this paper,the physiological response was studied to winter wheat varieties germination and seedling growth when different types of winter wheat varieties were under salt stress,And so the ameliorative effect of calcium nutrition on germination and seedling.The effects of different wheat genotypes and calcium concentration when they were under salt stress was also explored.Such as effects on wheat germination rate,the endosperm amylase activity when germinating,amino acid content and soluble sugar content,osmotic adjustment substance content, anti-aging enzyme activity,peroxide content and photosynthetic pigment content,and so on.The results indicated that:1.The effect of salt stress to buds of different winter wheat varieties.When under 1%NaCl stress,two different types of wheat germinating were inhibited,which showed in the germination rate,germination index, vitality index,the reduction on root number and length of bud.At the same time,the soluble sugar content in Wheat seed endosperm increased,while the amino acid content and amylase activity decreased.Whether rising or declining,the changing range of DK961(high salt-tolerant) was lower than that of JN17.2.The effect of exogenous calcium on different buds of winter wheat varieties when under salt stressAdditional exogenous calcium was helpful to the germination and eased the salt stress when the two different types of wheat varieties were under salt stress.The additional exogenous calcium on JK17(popular winter wheat varieties) were more apparent than that on DK961.Calcium concentrations in the 0.1%-0.4%range,the ameliorative effect increased at first and then decreased with the increase of calcium concentration.The effect of calcium concentration of 0.1%was the strongest for DK961,while for JN17,calcium concentration of 0.2%was the strongest,Calcium concentrations of 0.4% added salt injury to all of the varieties.3.The effect of salt stress on seedling of different winter wheat varieties1%NaCl stress might inhibit the growth of two different types of wheat seedlings.Which showed in decrease on Seedlings fresh weight,dry weight and water content by standard,and so photosynthetic pigment content;while the content of osmotic adjustment soluble substances such as sugar,soluble proteins,free amino acids and proline increased,the content of peroxidation product as MDA increased greatly,and so the activity of antioxidant enzymes such as superoxide of dismutase,peroxidase,catalase activity increased. Whether rising or declining,the changing range for DK961 was lower than that for JN17,which showed that the reaction to salt stress for DK961 was slower than that for JN17.4.The exogenous calcium on different seedlings of winter wheat varieties when under salt stressAdditional exogenous calcium was helpful to the seedling's growth and eased the salt stress when the two different types of wheat varieties were under salt stress.The ameliorative effect of additional exogenous calcium on JK17 (popular winter wheat varieties) was more apparent than that on DK961, which showed that the Seedlings fresh weight increased,and so the photosynthetic pigment content.The content of osmotic adjustment soluble substance and MDA(malondialchehyche) decreased,while the activity of antioxidant enzymes increased.Calcium concentrations in the 0.1%-0.4% range,the ameliorative effect increased at first and then decreased with the increasing of calcium concentration.The ameliorative effect of calcium concentration of 0.1%was the strongest for DK961,while for JN17,calcium concentration of 0.2%was the strongest,and Calcium concentrations of 0.4% added salt injury to all of the varieties. 5.The protect measures on germinating and seedlings in saline fields when wheat seeds were under salt stress.We should choose salt-tolerant wheat varieties,with the use of exogenous calcium.The calcium concentration was decided by different salt-tolerant wheat varieties.