Analysis Of The Expression Of The VDAC And ANT Gene Families

In this article, three potential ant and eight potential vdac gene sites, forecasted by our laboratory earlier, in rice genome were conformed, the expression of all of them in japonica rice, Nipponbare, and in indica rice, honglian cytoplasmic sterile rice YTA and YTB, and their distribution in wild rice and cultivated rice (O. sativa, AA; O. glaberrima, AA; O. officinalis, CC; O. punctata, BB, BBCC; O. punctata, BBCC; O. schweinfurthiana, BBCC; O. alta, CCDD; O. latifolia, CCDD) were analyzed. The major results as follows:(1) The three potential ant and eight potential vdac genes conformedBased on the potential ant and vdac gene sites forecasted by our laboratory using bioinformatics, the specific primers for the potential gene sites were designed for amplifying the sequence from rice genome DNA by PCR. By the sequence analysis of amplified DNA fragments, all the potential forecasted gene sites in rice genome were conformed.(2) The expressions of vdac and ant genes in seedlings of Nipponbare, YTA and YTBRT-PCR showed that all of the three potential ant and eight potential vdac genes expressed in the leaf, root and stem of Nipponbare, YTA and YTB seedlings. Real-time quantitative PCR showed that ant1 and ant2 perform a high level of expression than that of ant3, and the vdac8 has the highest expression among the eight vdacs, the expressions of vdac1, 2, 7 are a little higher than those of vdac3, 4, 5 in the tissues of three rice seedlings. The real-time quantitative PCR analysis also showed that the expressions of most of the genes tested in seedlings of japonica rice Nipponbare are higher than those in indica rice, YTA and YTB, and the expressions of ant and vdac genes varied between honglian cytoplasmic sterile line, YTA, and their maintenaner line, YTB, indicating the nuclear gene expression could be affected by their cytoplasms.(3) The distribution of the three ant genes and eight vdac genes in O. officinalis similar groupSix wild rice (BB, CC, BBCC, CCDD) and two cultivar rice (AA) genome DNAs in O. officinalis similar group were used for studying the distribution of the three ant genes and eight vdac genes by comparing the amplified bands with their specific primers. The results showed that vdac1, 4, 7, 8 and ant1 genes, which could not detected in DD genome, existed in AA, BB and CC genome, vdac6 gene existed only in AA genome, vdac2 gene presented in AA, BB and DD genome. vdac5 and ant2 genes cloud amplify in O.alta but not in O.latifolia, though they both were classified as CCDD genome, indicating the wild rice, O.latifolia and O.alta from Latin Amercian might be different.In this study, the confirmation of the three potential ant and eight potential vdac genes would be very useful for their cloning later; the expression analysis of these genes may provide some speculation for their physiological functions in rice growth and development; the distribution analysis of the three ant and eight vdac genes in O. officinalis similar group may provides very information for characterizing their evolution relationship.