Studies On Tissue Culture And Total Alkaloid Accumulation Of Fritillaria Przewalskii Maxim.ex Batal

The paper studies on total alkaloid accumulation and the tissue culture of Fritillaria przewalskii F. Przewalskii Maxim . The results are as follows:1. by The suitable hormone for Fritillaria przewalskii F. Przewalskii Maxim induced bulbs is MS + + NAA2mg / L +2,4-D2mg / L + 6-BA0.5mg / L + IBA0.5mg / L + KT0.2mg / L, the most appropriate temperature is 20℃, the most appropriate light is the darkness.2. The suitable hormone for Fritillaria przewalskii F. Przewalskii Maxim proliferation of bulb is MS + NAA1 mg / L, the most appropriate temperature is 15℃, the most appropriate light is the dark .3. The suitable hormone for Fritillaria przewalskii F. Przewalskii Maxim seedling is MS + NAA0.2 mg / L +2,4-D0 .5 mg / L +6- BA1 mg / L, the most appropriate temperature is 20℃, the most appropriate conditions are semi-light illumination.4. The suitable hormone for Fritillaria przewalskii F. Przewalskii Maxim seedling rooting is MS + NAA0.5 mg / L + IBA0.2 mg / L + VB1 mg / L, the most Suitable temperature is 15℃, the most appropriate light is the darkness.5. The total alkaloid content of regenerate Fritillaria przewalskii F. Przewalskii Maxim is higher than the total alkaloid content of natural Fritillaria przewalskii F. Przewalskii Maxim .6. The appropriate harvest time for regenerate Fritillaria przewalskii F. Przewalskii Maxim is 60 days.7. The suitable light for producting of Fritillaria przewalskii F. Przewalskii Maxim's total alkaloid is dark.8. The suitable temperature for producting of Fritillaria przewalskii F. Przewalskii Maxim's total alkaloid is 15℃.