Depict The Fingerprint Bands Of Kenaf Hibiscus Cannabinus L. And Castor Ricinus Communis L. With ISSR And SRAP Molecular Marker

Kenaf Hibiscus cannabinus L.and Castor Ricinus communis L.are both economic crops in china.In order to further the research on gerlnplasm resources and build the data base of DNA fingerprints bands for gene resources,this paper adopts 84 Kenaf and 81 Castor variety resources which are introduced and preserved from home and abroad as materials for experiment, then starts to screen ISSR and SRAP polymorphic primers based on the work of extracting DNA bands.In addition,it becomes to respectively PCR amplify as well as mark and analyzes using the screened combination of 28 ISSR primers and 26 SRAP primers with good polymorphism, build the excel of the data base for PCR amplified bands fingerprints of the Hibiscus cannabinus L.and Ricinus communis germplasm materials for experiment.According to the principle of uniqueness of the fingerprints bands,this paper adopts the self-designed software of analyzing on data of DNA fingerprints and then depicts the fingerprints bands of materials for experiment. Results are as follows:1.Analyze of ISSR molecular marking on Kenaf germplasm resources:it uses 84 Kenaf germplasm as materials for experiment,then screen 28 polymorphic primers from 80 ISSR primers such as UBC807-UBC890,and amplifies 84 Hibiscus cannabinus L.germplasm resources by PCR.It can amplify 230 bands,and each polymorphic primer can amplify 6.61 bands on average,including 185 polymorphic bands whose proportion is 80.43%,which indicates the abundant genetic diversity of Hibiscus cannabinus L.germplasm resources preserved in China.2.Depict of DNA fingerprints bands on Hibiscus cannabinus L.germ plasm resources: based on PCR amplified bands for genetic diversity ISSR polymorphism primers of 84 Hibiscus cannabinus L.germplasm resources,it screens five polymorphic primers as 813,825,836,888,889 from 28 primers with good polymorphism using analyzing software of DNA fingerprints bands which is programmed and based on image manipulation,then depicts the DNA fingerprints bands of 82 varieties of Hibiscus cannabinus L.,which can be regarded as molecular identification of fingerprints bands on Hibiscus cannabinus L.varieties.3.Analyze of SRAP molecular marking on Ricinus communis germplasm resources:it uses 81 Hibiscus cannabinus L.germplasm resources,then PCR amplifies the materials for experiment from screenedv 20 polymorphic primers from SRAP polymorphism upper and lower combination.It can amplify 263 bands,and each polymorphic primer can amplify 10.7 bands on average,including 214 polymorphic bands whose proportion is 81.37%,which indicates the abundant genetic diversity of Ricinus communis gennplasm resources preserved in China.4.Depict of DNA fingerprints bands on Ricinus communis germ plasm resources:based on PCR amplified bands for genetic diversity SRAP polymorphism primers of 81 Ricinus commnunis germplasm resources,it screens eight polymorphic primers as M8E10,M1E7,M6E3, M4E14,M1E11,M6E9,M16E17 using analyzing software of DNA fingerprints bands which is programmed and based on image manipulation,then depicts the DNA fingerprints bands of 70 varieties of Ricinus communis,which can be regarded as molecular identification of fingerprints bands on Ricinus communis varieties.Adopting the auto-recognizing and auto-analyzing DNA fingerprints bands system designed on the principle of uniqueness,this research primarily probes and attempts to depict the fingerprints bands of Hibiscus cannabinus L.and Ricinus communis germplasm resources for the first time.It is proved to be feasible and reliable,which is also the working effect and innovation of this research.