Establishment Of Molecular Identity Of Kenaf (Hibiscus Cannabinus L.)using Rapd, ISSR And SRAP Markers

Kenaf (Hibiscus cannabinus L.), annual herb of Malvaceae Hibiscus , is one of the most important bast fiber crops. Scientific and accurate identification of the genetic characteristics of kenaf germplasm has great significance to conservation and utilization of kenaf germplasm. It also means a lot to the identification of super genes, evaluation genetic resources, identification of seed quality and variety rights protection. In this paper, on the basis of morphology Investigation, 51 kenaf from different countries and regions with cultivated, wild and related species were analyzed by RAPD, ISSR and SRAP markers. Genetic similarity were calculated and Molecular identification were established. The results are followed:(1) The genetic similarity coefficient is 0.58 to 1.00 using morphological markers identified 51 varieties of kenaf germplasm. They are divided into four major groups at the genetic similarity coefficient of 0.74. The first group included all the cultivated species and wild species, and one related species 85-288 which was H.vitifolius, with close relationship between cultivated species. The second group formed by H020 ,one of the related species; The third and the fourth groups were composed of H.sab.var.altissim and H.sabdariffa.(2) Polymorphism rate (PPB) based on RAPD was 94.92%. 51 kenaf germplasm are divided into five major groups at the genetic similarity coefficient of 0.77. The first group included all the tested cultivated species and a closely related species,85-288. The second group were all tested wild speices. The third, the fourth and the fifth groups were related species. PPB based on ISSR was 89.38 %. The materials were divided into six groups at the genetic similarity of 0.74. The first group contained cultivated species and 85-288; wild species distributed in the second group and the fourth group. The third, the fifth and the sixth groups were composed of related species, including H. sab.var.altissim, H.sabdariffa and H.costatus. PPB based on SRAP was 98.8%. At the genetic similarity of o.71, the materials were divided into five groups. The first group were cultivated species, related species 85-288, 85-245 and wild species ZB90. The second and the third groups were composed of wild species.The fourth and the fifth groups were related species.(3) 20 RAPD primers produced a total of 10 typical bands, which could be able to identify 83-237., H180, H020 and Zaoshu Hongma; 19 ISSR primers generated 4 typical bands ,which could identify 85-235, Hongma 8 hao, Fujian meiguima and Qingpi 3 hao; 12 pairs of SRAP primers with 39 typical bands could effective identify 14 species.(4) Selected RAPD, ISSR and SRAP primers amplified rich bandmaps with high polymorphism. 18 cultivated species, 4 wild species and 10 related species were identified by RAPD markers. S1160 No specific bandmaps were amplified by S1160. 18 cultivated species, 4 wild species and 7 relatives species were identified by ISSR markers. U810 and U815 did not amplify specific bandmaps. 20 cultivated species, 4 wild species and 10 related species were identified by SRAP markers.(5) RAPD, ISSR and SRAP could effectively established molecular identities of the 51 materials with combination of typical bands, specific bandmaps of single primer and combinations of different primers. Three kinds of molecular markers clearly revealed genetic relationships among the Kenaf germplasm at the molecular level,which could provide the theoretical base for genetic improvement and selection of cross-bred parents in Kenaf breeding. It also could lay molecular biological foundation for variety identification , genetic improvement and Marker-assisted Selection in Kenaf.