Initially Investigation On Characters And The Curative Effect Of The Traditional Chinese Medicine For Histomonas Meleagridis

1. The eggs-water mixture of the Heterakis gallinarum were makedand the experiment animals were affected.The Heterakis gallinarum eggs was collected from the chicken cecum everywhere, the insect was broken and the eggs were cultivated in the air incubator,the eggs-water mixture were perparated, then, the experiment animals were Inoculated with 3 different density of the mixture. The Result is indicated that the Heterakis gallinarums and there eggs were easily to be find in those chicken which could find food by themeself ,.when the experiment aniamals were affected ,the group of high density and the group of middle density were got the complaints on the 10^th, but only one of the low density group was got the complaints on the 12^th .So, the right density was 2000 eggs in 1 mL water ,the middle density .2. Initially investigation on Characters of Histomonas meleagridisThe 18S rRNA gene sequence of histomonas was amplificated form the chicken's cecum which got the histomonosis with the primers HISF (5'-TTA GAA GTA GGA CCT TAG AGA-3') HISR(5'-TTA TAA GAG TAG CTT TTC ATT TT-3'), after amolificated if the gene strap which was wanted was just 491 bp ,the gene mixture must have the histomonas meleagridis's gene. A new method was found to diagnose the histomonosis. The method was used to diagnose 15 essaies from 4 areas, and the sickness rate from the 4 areas were 0.108%,0%,0%,0.015%.3. The curative effect of composition on HistomoniasisUsed the medicine of coptis Chinensis, baical skullcap root, amoorcorn tree bark, artemisiae capillaries to decoct at the appreciable proportion ,which need decoct the 3 times. Then, put the physicliquor condense into 100% oral administration liquid .The infected fowls of Histomoniasis were fed with 3 ways different dose.Tesult shown that the effective power to the infected fowls of Histomoniasis were 96.7% in high dose, 96.7% in middle dose, 90.0% in lose dose and the middle dose was the best amount of cure through the contrast with control group.