Screening And Matching Of High Efficient Rhizobium Strains Related To Pea Varieties

The test aimed at screening and matching of Rhizobium strains related to local pea varieties was conducted in Dingxi city in 2007,The nine Rhizobium introduced from Microbial Strains Storage and Management Centre of the Chinese Academy of Agricultural Science.and Yannong 2 and green pea are the two major pea varieties in Dingxi city in Gansu province, In this study, combining pot experiment with field test, the impact on the growth, nodulation of nitrogen fixation, yield and its factors of different varieties were studied. the major conclusions as following:In the same environmental conditions, Rhizobium and pea varieties exist symbiotic diversity and specific selectiveness, for Yannong 2, strains ACCC16103, ACCC16101, ACCC16110 and ACCC16082 are the best, while for Green Pea, ACCC16110, ACCC16103, ACCC16082 and ACCC16063 strains are efficient, And their individual total nitrogen content compared with their respective control increased 62.25%, 60.60%, 29.47%, 27.15%, 44.44%, 43.39%, 40.00% and 39.36%, respectively, the efficiency of nitrogen-fixing increased 38.37%, 37.73 %, 22.76%, 21.35%, 30.77%, 30.31%, 28.57% and 28.25% than their control, ACCC16103, ACCC16110 and ACCC16082 had broad-spectrum, adapting to two pea varieties efficiently. ACCC16101 is highly effective on Yannong2, ACCC16063 is a high-performance strain for green peas; the symbiotic effectiveness of different strains with different pea varieties had a significant difference. Under low nitrogen content condition, the Symbiosis of Rhizobium and pea can meet the nutritional needs of plants through nitrogen fixation. No matter which Rhizobium strains inoculated can increase the number of nodules per plant,tumor dry weight per plant and full nitrogen content per plant; can improve the efficiency of nitrogen fixation. However, for the characteristics of nitrogen-fixation and nodulation varied when different varieties inodulated with different Rhizobium. the impact of nitrogen-fixing Rhizobium the major factors which affected the nitrogen content are the number of nodules and tumor dry weight per plant, and they are significant positive correlation.Different Rhizobium for two varieties both had positive impacts on pea growth, which was different at growth cycle. High efficiency strains the positive role in pro–growth is more than that of post-growth, because the root did not invased by Rhizobium at branching stage, and it was still not affecting at budding time; at flowering stage, it was the key stage for pea growth and stage for Rhizobium activity. At this time,the impact on plant height, stem, leaves, overground part fresh weight and dry weight is significantly better than that of the control. Observation from the entire growth cycle, the treatment inoculated with the fine strains are not only with deep color, flourish leaves, tall and stout plants, and also with numerous nodules and tumors. For Yannong 2, the strains with better performance are ACCC16101 (B2), ACCC16103 (B5) and ACCC16082 (B8). The number of nodules, tumor dry weight per plant, overground part plant dry weight, fresh weight of root, root dry weight and nitrogen content of plants are superior to other treatments; while for green peas, the strains with better performance are ACCC16082 (B8), ACCC16110 (B3), ACCC16101B2 (B2) and ACCC16063 (B9), the above indicators are also better than the other treatments. Field test results showed that for Yannong 2, the strain with better indicators such as the number of nodules, tumor dry weight per plant, overground part plant dry weight, fresh weight of root, root dry weight and nitrogen content of plants is still the ACCC16101 (B2), which means optimized strains that have a certain stability. Compared to pot test it had a better yield consistency.The strains which can match perfectly with pea varieties, adapting to local ecological environment improved branches per plant, grain yield per plant and 1000-grain-weight, while had few the number of stems grains and pods per plant, and had no impacts on overground part fresh and dry weight at full-bloom stage, but in the maturity had a significant impacts on plant biomass. vaccination efficient of Rhizobium can effectively improve economical outputs and water use efficiency. ACCC16101 has positve influence on economical yield, nodulation status, the growth momentum than other strains. The yield increased 48.76%. despite the strain ACCC16103 did not show the same yield increasing effect like strain ACCC16101, but the nodules dry weight, the number of nodules per plant and the total nitrogen content compared to the control increased by 82.61%, 49.09% and 40.74%. Primarily, For Yannong2,it was believed ACCC16101 and ACCC16103 were preferred bacterial strains for rhizobium inoculation and rhizobium inoculants production.