Study On The Expression Of IL-10 In Brain And Colon Tissue Of Rats With Inflammatory Bowel Disease

Inflammatory bowel disease (IBD) is an important intestinal disease, including the crohn disease (CD) and ulcerative colitis (UC).There is a great diversity of clinical symptom, a bad therapeutic efficacy and an easy recidivism. Especially in Europe and the America, it gets a high morbility, simultaneously, the morbility is increasing gradually in recent years. The etiopathogenisis and nosogenesis are unclear now. Most researchers think they are related with infection, immunologic abnormality, heredity and so on. Immunologic abnormality is considered to play the most important role among all of these factors.T cells play an extremely important role in the immune system. According to the difference of cytokines that CD4 +T cell secretes and mediating immune function, they are divided into Th1 and Th2, Th1 cells express IFN-γand IL-2 mainly, and Th2 cells express IL-4, IL-5 and IL-10 etc. More and more evidences prove that Th1 and Th2 have different roles in the course of mediating immune response. Th1/Th2 imbalance has been considered to be one of the pathogenesis of IBD. When Th1 or Th2 cells enhance mucosa inflammatory response, the amount of the suppression cells, regulatory T cells and the secretion of cytokines decreased significantly, which demonstrated in the development of inflammatory bowel disease the imbalance plays an important role. In short, both T cells and their sub-type (Th1,Th2,Th3) and cytokines (pro-inflammatory cytokines, anti-inflammatory cytokines and growth factor) play a significant role in the pathogenesis course of IBD immunity.Nervous, endocrine and immune form a complex network, named neuro-endocrine-immune regulatory network, which controls the body's various vital movement, maintains homeostasis of the body. When the homeostasis is disrupted, certain parts in the network will respond and do some change in the function, meanwhile, some change occurs in the referred network, leading to certain diseases and the emergence of pathological process. Neuro-endocrine-immune disorder is a common feature of all the diseases. All the clinical diseases have a nerve-endocrine-immune disorder. The disease process is actually a kind of disorder for homeostasis regulation of nerve-endocrine-immune network, however, among the nerve, endocrine and immune disorders, immune disorder is principal. Since the occurrence and development of IBD are closely related to T cells and their cytokines, the nerve - endocrine - immune regulatory network plays an important role in regulating T-cell-mediated immunity. However, whether the nerve-endocrine-immune regulatory network mediates the development and occurrence of IBD, few are reported today.In view of the above, the experiment is to use artificially induced IBD rats as models, make use of immunohistochemical method, to discuss the effect and mechanism of IL-10 in the occurrence and development of IBD, from the view of the nerve-endocrine-immune network and the immune homeostasis regulation. It will lay a strong foundation to ensure the relationship of the course of IBD and neuroendocrine immune network, provide a new way to prevent and treat the inflammatory bowel disease (IBD) , and offer experimental basis for further study at nerve endocrine immune network theory. Therefore, we make IBD models by giving an enema with trinitro-benzene-sulfonic acid. Put the IBD rats to death on the 3rd ,7th ,14th ,21st, 28th day. Take the brain and colon tissues. Detect the expression level of IL-10 in the intestinal canal and brain by immunohistochemistry and acquire the concentration of IL-10 in the serum by ELISA. Results show, with the development of IBD, there is a lower expression level of IL-10 in the colon. When rats recover, the expression level of IL-10 increases gradually, and finally reach the normal level. At the same time, the expression level of IL-10 in the cerebral cortex and hypothalamus has a opposite change during the whole process of the IBD. The concentration of IL-10 in the serum begins to lower when IBD happens, on the contrary , it begins to increase accompanied with diease recovery. The experiment shows that IBD is closely related with the activity of neuroendocrine. Immune-nerve-endocrine network may be involved in the process of the occurrence and development of IBD, and it plays a role in the pathogenesis of IBD. Experiment provides a theoretical basis for the study of pathogenesis.