Isolated Of The Penicillium Cyclopium Westling In The Feed And Identification Of The Extraction

Penicillic Acid(PA),which is mostly producted by Penicillium cyclopium,PA major pollution wheat,rice,corn and other agricultural products and animal feed,in feed can achieve the highest level of 2%.PA is one of the common mycotoxin,which is harmful to the agriculture industry.Long-term feeding with PA feed,make animal hepatomegaly,hepatic cell degeneration and animal cells can inhibit DNA synthesis, serious can make animal cell DNA breakage.PA can produce joint toxicity with other mycotoxin and PA could synergism with other common mycotoxin may have greater toxicity and threat to human and animal health.Therefore,studying the Penicillium cyclopium and PA in feed would make great significance sense to enhance the quality of livestock products and the security of food.Purpose:Through the isolated culturing and appreciation of Penicillium cyclopium and the extraction,depuration,and appreciation of the Penicillium cycopium metabolic product-PA,We attempt to establish a method and theory for the toxicity studying and fast-detection of PA in this research.Method:The first suspicious mouldy feed to culture,feed mold growth in the future followed by a separate inoculation training,according to a separate vaccination fungal growth under mature beyond the size,color,shape preliminary classification.After the mold separate classification will be inoculated in high salt liquid Czapek's Medium glass coverslips,at 25℃feed mold incubator cultured 3 to5 d,wait until there are spores sprouted after Dyeing is observed under the microscope mold spores and hyphae of the morphological structure.Penicillium cyclopium spores will be inoculated in 25℃feed mold incubator cultured 14 d,chloroform extract toxins in the feed.Chloroform extract concentration after column chromatography,identified the fractions by TLC, determined purpose component through HPLC-MS Spectrometry to further define the content of Penicillium acid. Result:1.We isolated 6 kinds of Penicillium from 21 moldy feed,they are P.cyclopium Westling,P.citrinum Thom,P.funiculosum,P.expansum,P.claviforme and P. crustosum.2.We have extracted and isolated the metabolic product-PA from cultured Penicillium cyclopium.Conclusion:Penicillium pollution is the major pollution of feed,and the major metabolic product of Penicillium cyclopium is penicillic acid.Separated and identified 6 kinds of Penicillium from the feed,with collected from Hunan Province.White Penicillium cyclopium metabolite is PA,which identified by thin-layer chromatography and HPLC-MS seized analysis system.