| Watermelon is a natural and rich source of beneficial secondary plant substance for human.Such as Lycopene,L-citrulline,Vc.Many watermelon varieties include diploid, triploid and tetraploid were employed to study The lycopene,L-citrulline and Vc contents and its changes during watermelon fruits development.It served as a theoretical fundamental and guide of breeding and cultivating watermelon with high lycopene, L-citrulline and Vc contents.1 A discuss about the method of L-citrulline determining in watermelon friuts:In this study,the extracted technology and spectrophotometry determination of citrulline in watermelon were established.The results showed that the highest efficiency of citrulline could be extracted with MeOH:6MHCl(v/v=9:1)with active carbon 10g in a bath for 20 min,followed by heating at 55℃.1:1.5 radio of sample and extractor,,.According to the special color development reaction between L-Citrulline and Diacetyl monoxime in the strong acid solution and the linear relationshio feature which reactive compoud color varied with L-citrulline consistency.The precise,convenient and sensitive method of The pectrophotometry determination of citrulline was set up.2 The changes of functional ingredient in watermeon fruits development:A total of 2 watermelon(Citrullus vulgaris) cultivars(FANZU NO.2,XQ,M2) include 2x,3x,4x were selected.The lycopene,L-citrulline and Vc contents changes were determined during their fruits development.All changes curves of functional ingredients contentsof three ploidy cultivars were similar.The lycopene content were very low(0.2～0.6mg.100g-1)after 15 days of artificial pollination The 25～28 days after artificial pollination was the period of lycopene sharp increasing and it was the highest in ripe period.The changes in overripe watermelon decreased slowly.The L-citrullline content were very low after 15 days of artificial pollination.The 20～28 days after artificial pollination was the period of sharp increasing and it would reach the peak value at 31～34 day after artificial pollination.It began to fall slowly after 37～40 days after artificial pollination.The Vc contents in young fruit and maturation in watermelon were higher.The lycopene,L-citrulline and Vc contents in 10 ripe fruits include diploid,triploid and tetraploid was determined.The results showed that The lycopene,L-citrulline and Vc contents contents were significant difference in different ploidy watermelon.The lycopene,L-citrulline and Vc contents in triploid or tetraploid were higher than in dipoidy in eight cultivars.It is significantly differenent(P＜0.05).3 The difference of functional ingredient in different ploidy watermelon:A total of (Citrullus lanatus)Cultivars were selected.The lycopene,L-citrulline and Vc contents in 10 watermelon ripe fruits include diploid,triploid and tetraploid was determined.The results showed that The lycopene,L-citrulline and Vc contents were significant difference in different ploidy watermelon.The lycopene,L-citrulline contents in triploid or tetraploid were higher than in diploid in eight cultivars.The Vc contents in triploid or tetraploid were higher than in diploidin nine cultivars.It is significantly differenent(P＜0.05).4 The difference of functional ingredient in different colour flesh:Many different colour flesh with same ploidy watermelon(Citrullus lanatus) cultivars were selected.The lycopene,L-citrulline and Vc contents of different colour flesh in ripe fruits of their diploid,triploid and tetraploid were determined.The results showed that The L-citrulline contents of yellow flesh was more than that of red flesh in diploid and triploid watermelon. The results in tetraploid is oppisite.The Vc contents of yellow flesh in diploid is the most, the second is white flesh and the third is red flesh.The Vc contents of yellow flesh were more than that of red flesh in triploid and tetraploid.The lycopene contents of yellow flesh were lower than that of red flesh in diploid triploid tetraploid.The Lycopene of white flesh in diploid is zero.
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