Differential Proteomics Analysis Of The Brucella Melitensis A Virulent Strain 16M And Vaccine Strain M5

Objective:To analyze different expression proteins of the brucella melitensis a virulent strain 16M with vaccine strain M5 by the technologies of proteomics, and explore the mechanism of virulence in B .melitensis and find out the diagnosis markers of the brucellosis.Methods:Extracted total proteins of the brucella melitensis a virulent strain 16M and vaccine strain M5 by acetone cryochem,separated proteins by two-dimensional electrophoresis(2-DE),stained proteins by Coomassie brilliant blue, got the patterns,found out differential proteins, and obtained PMF(peptide mass fingerprinting) by MALDI-TOF-MS, gained related the information of proteins by database.Results:In this study, 2-DE Patterns with high resolution and reproducibility from the proteins of the strains 16M and M5 was obtained. The total Protein distribution pattern of the strains16M and M5 2-DE is very similar.A total of 856 and 793 protein spots were detected for strains 16M and M5,respectively.20 out of 23 protein spots were identified by the technologies of mass spectrogram with bioinformatics.20 protein spots identified by the technologies of mass spectrogram with bioinformatics seem to play important roles in a variety of pathways including sugar binding, protein production,lipid metabolism,and molecular chaperone.13 of 20 are upexpressed proteins in strain 16M,5 of 20 are upexpressed proteins in strain M5.