Controls For Callus Differentiation And Preliminary Study On The Mechanism In Indica Rice (Oryza Sativa L.)

Establishment of high efficiency transformation has greatly facilitated the widespread application of transformation in japonica rice. In contrast, there have been very few successful cases reported for indica rice. Recently, through the seeds-derived calluses of indica rice, an efficient callus preservation system has been successfully established in Zhaoming Pan's laboratory.The utilization and molecular mechanism of this method will be uncovered in this study. It was found that the frequency of callus differentiation going a stable state along the time, which was high, feasibility of long-term callus persevered was carried out. Up to now, embryogenesis callus have been cultured for 15 months, which were light yellow, drying, compact and with high differentiation rate compared with those of the control. The callus would start green in a short time after transferred to regeneration media, bud in two weeks and regenerate quantity of caespitose seedlings, which rate was high to 99.8%. And there was no obvious ill varitiation in the development of the seedings. In addition, different varieties of anther and seed were used for callus induction to validate the applicability on callus from different explants and genotype. It turned out that it was not the behavior of the callus from special explant or genotype, but the treat method induced the long-term preservation of callus. Synchronously, the proteins of the callus were analyzed by SDS-PAGE, some special proteins were separated from the callus, and the molecular weight of which were about 40KD, 42KD and 48KD.