Studys On The Ginsenosides Microbiological Transformation Of The Fruits Of Panax Ginseng C.A.Meyer

The fruits of Panax ginseng C.A.Meyer,with the high ginsenosides content and the evidently pHarmacological activity,has been studied extensively along with the research development on the other part of the Ginseng's plant except the root.But up to now,there were a few reports on the chemical studies of the fruits of Panax ginseng.This research takes the fruit of valuable traditional Chinese drugginseng as raw material,The biological system chooses,Mainly by Geotrichum candidum and so on 5 kind of strains primarily,In the substrate choice aspect,Then take the ginseng soap glucoside in certain monomer soap glucoside content big ginseng fruit juice as the first choice,The rocker liquid fermentation research ginseng soap glucoside biotransformation report are few,Above mold mushroom spawn fermentation transformation ginseng's soap glucoside not yet saw me system until now the report. In recent years,along with molecular biology technology progress,The ginseng soap glucoside biosynthesis heavy causes people's research enthusiasm,and attempts from the gene level to expound the ginseng soap glucoside biosynthesis way.This experiment using the fruits of Panax ginseng juice and the fermentation fluid liquid fermentation method from the ginseng total soap glucoside content change,the monomer soap glucoside composition proportion and so on the aspects to conduct the systematic research.Antiseptic turns on the Geotrichum candidum the fruits of Panax ginseng C.A.Meyer juice after the cobalt 60 radiations and so on 5 kind of strains to carry on the microorganism fermentation separately,Determines the best fermentation process condition through the orthogonal experiment,most suitable pH is 7.0,the best attire liquid volume is 120ml,the optimum temperature for 30℃,the most suitable rotational speed is 120 rpm.Obtains the ginseng fruit juice fermentation fluid,the total soap glucoside content highest is ferments 14 day-long Geotrichum candidum,mainly by white mildew and so on 5 kind of strains primarily,after the fermentation achieves 4.366mg/ml,the total glucoside content outdoes the fruit juice raw juice 22.47%.Concentrates the fruits of Panax ginseng total soap glucoside through the pocket adsorption resin column,through static adsorption experiment to 4 kind of pocket adsorption resin coarse screen,designated adsorptive capacity big resin D101.And carried on to resin D101 has adsorbed pH,the analysis ethyl alcohol density,analyzes the pH value and so on to carry on the preliminary discussion,has obtained the best craft:Ginseng fruit juice fermentation fluid pH6.5 achieved the biggest adsorptive capacity,take PH as 7.70%ethyl alcohol is the eluent,the speed of flow is 6ml/min may achieve the biggest analysis rate 56.89%organic solvent extractions,the extract,the pocket adsorption resin chromatograpHic analysis,conventional and so on silica gel column chromatograpHic analysis pHytochemistry methods and the technical method through many kinds of different extractions,the separation craft route,obtain 5 monomer compound from the ginseng fruit,and unifies the HPLC method,the monomer soap glucoside carries on the content determination to in the ginseng fruit juice raw juice and the fermentation fluid.These work completion,provides the new method,the new way for the ginseng soap glucoside biotransformation and the biosynthesis,to the thorough research ginseng soap glucoside biotransformation,the biosynthesis mechanism,the new use development has the big theory significance and the practical application value.