Study On Gene Engineering For High α-Linolenic Acid In Rapeseed (Brassica Napus)

α-linolenic acid(ALA;C18:3Δ-9,12,15) is a nutritionally important polyunsaturated fatty acid for human.However,ALA sources are not ideal for dietary supplementation due to large fluctuations in availability.As a result,there is a considerable interest in the large-scale production of ALA by transgenic oil crops.My research is focused on introducingΔ12-fatty acid desaturase gene(FAD2)andΔ15-fatty acid desaturase gene(FAD3) to conventional oil crops(rapeseed) in order to produceα-linolenic fatty acid for functional foods and pharmaceutical products.The upstream regulatory region ofΔ12- fatty acid desaturase gene,Δ15- fatty acid desaturase gene and a seed-specific gene was isolated from the genomic DNA of B. napus "Zhongshuang 9" by RT-PCR and PCR amplification.The PCR products were cloned into pBI525 vector.Plant pZP212-FAD2 expression vectors containing CaMV 35S promoter were constructed.Plant pZP212-FAD3 expression vectors containing napin gene promoter were constructed.Then the gene of FAD2 and FAD3 were put into the same pZP212 expression vectors.Inoder to improve the lever of express,the MARs were constructed between the gene of FAD2 and FAD3.Agrobacterium-mediated transformation system for rapeseed(Brassica napus) using cotyledonary petiole as explants was optimized,bringing 16 plants(Zhongshuang 9) transformed with pZP212-FAD2-FAD3.