Cloning Of Special Expressed Genes And Promoter From Peanut Pericarp And Testa

Seed is the main target of the peanut. The quality and production of peanut has close relationship with it. But it is easily infected by Aspergillus flavus. The main object of this research are as follows:(1) isolating the full-length gene by constructing a full-length of pod and testa cDNA library. (2) Studying the expression pattern of those isolated genes and cloning their upstreaming promoters. This can provide us special espressed promoter for the transgene project of peanut. And this can make the foreign resistane gene's production expressed in testa and pericarp specially but not in seed. This can help us to solve the safety of genetically modified food in some extent.1. A pod and testa full-length cDNA library was contructed and three special genes were isolated by the PCR method. These genes were named AhPSG8, AhPSG13 and AhPSC11. The sequencing result showed that AhPSG8 contains 1118bp and the initial codon was from 33bp to 35bp. The open reading frame was from 33bp to 833bp and encoded 266 amino acids. AhPSG13 was 1369bp and the open reading frame was from 28bp to 1122bp, encoding 364 amino acid. AhPSC11 contained 609bp and the ORF was from 67bp to 462bp ,encoding a peptide that contained 131 amino acids. They were all novel genes and were submited to NCBI/GeneBank.2. We cloned the promoter by the method of chromosome walking on the basis of the full-length of these genes. Bioinformatics analysis showed that P-AhPSC11 was 683bp long and contained some domains that were related to transcription, such as TATA Box, CAAT Box and so on.