The Red Rice Character Genetic Analysis Of The Special Rice And It's Heterotic Vigor Utilizing

The high quality special rice including red rice, black rice and the like has abundant nutrition and microelement and the medicinal value. So they have a potential market occupy percentage. This paper deals with the rice color genetic analysis and it's heterotic vigor utilizing. The main results are summarized as blow:1.the location of color rice pigment in rice grainAt 21℃, the 100 red, white and black rice grains were soaked respectively in water. After two days, we could see three different colors: colorless in white rice solution, reddish orange in red rice solution and purplish red in black rice solution. Also with the electron microscope the rice pigment was found to be in the "testa and fruit-cavity of rice grain, the color in the testa was thicker than in the fruit-cavity and the thickness was different in the different the testa cells.2.the rice red character genetic analysis and the key controlling gene mapping Zhenshan97B, II-32B, D62B, G46B were positively and negatively crossed with ruby B and the F1 rice grains were red. That indicated the rice red character genetic had the testa and fruit-cavity xenia. The rice grain color of F2 was segregative: thick red, red (15) and white (1) under the effect of the polygene. By the X2 test of the 8 F2, the red character was found controlled by the two dominant key genes.By using the recessive white population made of 182 plants and 397 SSR primers, one pigment key gene was first found tightly linked to the RM212 on chromosome 1, and genetic distance is 9.6cM. We named this new mapped gene RRM.3.the restoring analysis of Ruby B and the fertility-restoring gene mapping To utilize the heterotic vigor, we succeeded in breeding the Ruby A by backcrossing based on the Ruby B. We found that the Fl population all is sterile but the BC1 generate fertility restoring, and the percentage of the sterile plants rising with the backcross frequency rising at the breeding process. The pollen fertility of the (zhenshan97A, D62A, G46A and D702A)/ Ruby B Fl,and F2 ,BC1 populations examined, the results showed one main fertility-restoring gene effecting the fertility's expression by the X2 test and the formulaK=(logm-logn)/0.6021.The controlling gene was liked to RM182 on the chromosome 7 and the genetic distance is 7.4CM. That was not the same as the usually fertility-restoring gene family location on the chromosome 10 so it was a special gene.Otherwise, the BC1 > BC2 and BC3 sterile plant of the Ruby B were analyzed by the RM182, and the result showed that the backcross based on the Ruby B time and again could breed the new sterile lines.4. sterile gene allelism analysisZhenshan97A, II-32A, D62A , G46A and Ruby A were crossed with the zhenshan97B, II-32B, D62B, G46B and Ruby B. That the all Fl pollens were almost sterile indicated the sterile genes of different kytoplasm sterile lines allelic. S.fertility- restoring gene allelism analysisBy crossing zhenshan97A, II-32A, D62A , G46A and Ruby A with Bg1639, R527, R881, 250(CDR22/Bg403), these restoring genes were found to be allelic. 6. the heterotic vigor analysis of Ruby ATo test the heterotic vigor of Ruby A, the Ruby B yield and Bg 1639s R527. R88K 250(CDR22/Bg403)XRuby A Flwere tested. The yield comparison between the Fl and Ruby B showed that Ruby A had a strong heterotic vigor and good combining ability for that the biggest F1 yield could add 159.5Kg.