Study On The Optimization Liquid Fermentation, Extraction Technology And Sulfate Modification Of Pachyman

Poria cocos is one kind of valuable medicial and edible fungus,and the polysaccharide form Poria cocos has a wide variety of medicinal activities,such as enhancing body immuneity,anti-tumor,and many other effects.With the rapid development of bio-molecular science,the function of polysaccharide in many biology is continently found and recognized.The study of polysaccharide drew interests,especially in chemically modifications of polysaccharide.In this paper,the experiment studied using liquid fermentation we got the pachyman.And find the liquid fermentation optimum conditios of pachyman;extraction optimum conditions of mycelium.A kind of sulfated polysaccharides was obtained and its structure was proved.There is a great significance to study on industrialization production of pachyman,expand the scope of application of pachyman and its derivatives.The main results were described as follows:1.The optimal ratio of carbon sources,nitrogen sources,was identified through a sing factor screening test.And studied the culture temperature,rotation speed,pH and inoculum amount.By the method of Orthogonal experiment,optimize the medium.The optimal medium ratio of the liquid fermentation was as follows:glucose 4%,peptone&KNO₃ 1.5%,pH 5.5,Liquid volume 80mL/250 mL,rotation speed 150rpm,temperature 26℃,inoculum amount 6%.2.Single factor experiment and response surface methodology were also successfully applied to optimize the operation parameters which affected the extraction yields of pachyman from the mycelia.The maximum extraction yields were 2.45%,when the mycelia were extracted with the following operational parameters:temperature 73.8℃,liquid-solid ratio 29.8:1,extraction time 4.3h.The R² value of model was 93.16%,also the independent variable had significant linear relations with attributive variables.Therefore this model could forecast the final content of pachyman during extraction abstractly.There is a single component by analysis after purified.3.The sulfated pachyman(SP) was obtained with chlorosulfonic acid-pyridine.The best conditions of synthesis through comparing the effects of differert conditions such as vol.proportion of chlorosulfonic acid-pyridine,reactive temperature and reactive time.Degree of substitution is 0.68.And identificate SP's physicochemical properties.Structure of SP was proved by UV spectrum and IR spectrum.The results indicated that synthesis of the sulfate is made.Identifide the molecular weight was about 5.4×10³ Da by Sephadex G-75 and agarose gel electrophoresis.The characteristic of SP synthesized in this paper includes homogeneous component,lesser molecular weight and more DS,it may be a more biology-action sample for many fields,such as anti-tumour and so on..