The Study Of Liquid And Solid-fermentation Process And Polysaccharide Content Of Hericium Erinaceus

Hericium erinaceus is a medicinal and edible mushroom, has a good anti-ulcer, anti-inflammatory, anti-tumor, liver protecting liver, lowering blood glucose and lipid effects. Hericium erinaceus polysaccharide is recognized as the main active ingredients with biological activity. Currently, the main polysaccharide from the fruiting bodies and submerged fermentation culture mycelium obtained. But the long period of fruiting body culture, liquid fermentation conditions difficult to control, parameter requires more prone to contamination. This study is the way to solid-state fermentation culture Hericium erinaceus the single factor and orthogonal optimal method of combining, respectively, sub-species of Hericium erinaceus liquid medium and solid medium were screened for optimization.Through the liquid carbon source, nitrogen source and pH and other factors, screening optimization, to finalize the liquid seed culture medium for glucose 2.5%, soybean meal 4.0%, MgSO4·7H2O 0.5 g/L, KH2PO41 g/L, pH5.0, medium volume 200ml/500ml,150r/m,25℃cultured for 10 days. Conversion rate of hyphae at this time 39.28%,1L medium can be 25.53g hyphae. On corn, potato residue, wheat bran and other matrix and matrix ratio, the ratio, inoculum and other factors, screening optimization, and ultimately determine the Hericium erinaceus is a solid state fermentation of corn matrix medium:potato residue=12:8, fill of load 20g, CaCO3 0.5% added, the ratio of 1:1, cultured for 11 days, then the conversion rate of 7.25% mycelium.This paper on the wild Hericium erinaceus polysaccharide, Hericium erinaceus polysaccharide fermentation liquid, solid-state fermentation of Hericium erinaceus polysaccharide extraction time, extraction temperature and solid-liquid ratio of the three factors were orthogonal three factors and three levels of optimization to determine the wild fruiting bodies of Hericium erinaceus polysaccharide extraction process for the extraction temperature 90℃, solid to liquid ratio 1/40, the extraction time 5h, extraction rate of about 5.496%; submerged fermentation process for Hericium erinaceus polysaccharide extraction temperature 90℃, solid liquid ratio of 1/50, the extraction time 3h, extraction rate was 1.615%; solid state fermentation process for Hericium erinaceus polysaccharide extraction temperature 100℃, solid to liquid ratio 1/40, the extraction time 5 h, extraction rate of 2.504% Wild Hericium erinaceus mycelium of liquid sugar is about 3 times about 2 times the solid-state fermentation mycelium.