Storage Of The Free Living Conchocelis Of Porphya Yezoensis By Encapsulation-dehydration Under Normal Temperature And Low Temperature

The diversity of germ plasm resource is important premiss to maintain the ecosystem balance, relate to the development of agriculture and the exist of human derectly. Since 20th century, The diversity of germ plasm resource have been suffering from destroyed,and the amounts was greatness. Therefore, the measure of how to protect the germ plasm resource not been destroyed was became a problem globality.Due to one part of plant, Storage of the algae have inportant signification.In the nature, the algae beening knowed was about 37000 kinds. They kind important count for economy. The algae was ont only edible,but also was the bait of marine product breed aquatics,fertilizer of the agriculture produce,material of the industry and medicament.The free living conchocelis of Porphya yezoensis Ueda 03B were preserved at normal temperature and low temperature by using an encapsulation-dehydration method. The free living conchocelis were encapsulated in 3% Ca-alginate beads with 30‰NaCl, Beads were desiccated with silica gel, then were preserved under normal temperature and low temperature. The main factors influencing the viability,such as temperature,light(dark) and water content of beads were studied. Result is indicated:1. The Effect of temperature on viabilityThe beads were preserved after several months, The viability of free living conchocelis was the best at 4℃,reached 51.6% after 18 months, The viability of free living conchocelis reached 42.8% at 20℃,but the viability was dropped rapidly with the preserved time. the lowest viability was storaged under -30℃without glycerin,only 9.3% after 12 months.2. Effect of light and dark on cell viabilityThe viability of free living conchocelis that stored at darkness was higher than that of free living conchocelis that stored at reduced irradiance. Thereinto the preserve under 4℃was the evidentest, the viability was dropped rapidly with the preserved time at reduced irradiance.3. Effect of water content on cell viabilityThe viability of free living conchocelis preserved under 20℃and at 4℃(reduced irradiance) was dropped with the water content dropped,but The viability of free living conchocelis preserved under -30℃and at 4℃(dark) was rised with the water content dropped. With the diffrence of water content, The viability of free living conchocelis preserved sevral months was different.4. Effect of glycerin on cell viabilityThe viability of beads that were incubated in culture medium containing glycerin higher than the beads without glycerin under -30℃. The viability of beads that were incubated in culture medium containing glycerin reached 53.4% after 12 months, The viability of the beads without glycerin was only 9.3%.5.The growth curve and configuration of the free living conchocelis By measuring the growth curve of the free living conchocelis after preserving ,discovered that it wasn't different from that of un-preserving.The free living conchocelis was Observed under the microscope,the configuration of the free living conchocelis after preserving wasn't different from that of un-preserving.Encapsulation-dehydration is simple without the requirement for specialist equipment, The viability of free living conchocelis was the best at 4℃,reached 51.6% after 18 months,so it has a bright application prospect in the algae preservation...