Studies On Establishment Of High Efficient Regeneration System And In Vitro Induction And Selection Of Salt-tolerance Mutants In Potato

In this research,the high efficient regeneration system of four potato cultivar was established by using four types of explants, stem tip stem leaves andr tube chip;the screeningt echniques for salttolerance and mutants of potato stem tip callus in vitro were studied, and primary screening system for salt tolerance evaluation was established;the effect of DPC on application in potato plantlets tissue culture. .The primary content and result are as follows:1. Regeneration medium of 4 potato varieties was screening.The optimal regenera -tion medium for stem tip was NAA0.1 mg/L+6-BA2.0 mg/L+KT0.05 mg/L+GA0.1 mg/L+CH 500 mg/L. The regeneration medium for stem were NAA0.01mg/L+ 6-BA2.0 mg/L+ GA5.0mg/L and NAA0.25mg/L + 6-BA1.5 mg/L +GA7 mg/L.The optimal regeneration medium for callus induction from leaves was NAA0.2 mg/L + 6-BA2.5 mg/L + GA10.0mg/L+AgN03 4.0 mg/L;The optimal medium for adventitious bud differentiation from stem-derived calluses was found to be 6-BA2.0 mg/L+GA10 mg/L+AgN03 4.0 mg/L.The optimal regeneration medium for tube chip was ZT2.0 mg/L+IAA1.0 mg/L+GA0.1 mg/L; adding 0.1 mg/L GA into medium suitable medium to potato tube chip differentiation.2. The optimum dose of 60Co-γrays treatment on callus induction from stem tip of potato.The 31 mutants were tolerant0.8%NaCl.The 17 mutants were tolerant 1.0%NaCl. After primary selection from critical content of NaCl, the induced mutants could resist salt stress by increasing CATase and content of free proline,and decreasing content of MDA,and Chlorophyll.The salt tolerance of induced mutation was higher that of control. Salt tolerant tests in vitro revealed that the mutants possessed salt tolerance and had genetic stability3. Suitable concentration DPC can be applied in multiplication , strengthening and preservation in vitro...