Screening Of Physiological Drought-resistant Lactobacillus And Research Of Low-moisture Silage Fermentation Additives

Eight bacterial strains with better growth and acid-production performance, NT1 to NT8, were separated from several excellent fermented dry forages and low-moisture silages at home and abroad. NT3﹑NT6﹑NT7 belonged to Lactobacillus Casei.After they were cultured in MRS liquid culture medium for 36h, pH value were 3.26,3.18 and 3.20 respectively; NT4 and NT8 belonged to Lactobacillus Maize. After they were cultured in MRS liquid culture medium for 36h, pH value were 3.35 and 3.31 respectively;NT2 and NT5 belonged to Lactobacillus Jensenii. After they were cultured in MRS liquid culture medium for 36h, pH value were 3.82 and 3.97 respectively; NT1 belonged to Lactobacillus Kimchi. After they were cultured in MRS liquid culture medium for 36h, pH value was 3.48. Four kinds of bacterium were combined with two, three or four and four combinations with relative high growth and acid production rates were selected, pH value were 3.27,3.40,3.19 and 3.13.Lactobacillus combinations and monomer from separation,as low-moisture silage additives, comparing with the control group, significantly improved fermentation quality of alfalfa, corn and a mixture of both, promoted a strong lactic acid fermentation, increased lactic acid content (p<0.01 ), and decreased pH value (p<0.01). The lactic acid content in low-moisture silage in which alfalfa was raw material reached to 2.65%FM which was 1.49% higher than the control group;and the lowest pH value may fall in 4.37, but the control group pH value was 5.10.The pH value of low-moisture silage in which corn was raw material dropped in to 3.7 which was 0.56 lower than the control group. NH3-N content in low-moisture silage in which alfalfa and the mixture were raw materials decreased significantly (P<0.01) which was probably 0.2～0.3 mg/g FM lower than the control group, while that of in low-moisture core silage did not changed significantly(p>0.05). WSC content could be significantly increased in corn and mixed low-moisture silage (p < 0.01), and WSC content in low-moisture corn silage reached to 72.93mg/g FM, while it did not change significantly in low-moisture alfalfa silage (p>0.05). There was no significant influence on CP content(p>0.05),but ADF and NDF content were improved. Grasses contained corn and such and Legumes contained alfalfa were mixed to be low-moisture silages with bacterial of treatment group 5 had the optimal effect by comprehensive analysis.The degradation rate experiments with sheep rumen nylon bag showed that, comparing the treatment groups with lactobacillus additives with the control group, the effective degradation rate of low-moisture silage DM in corn, alfalfa and a mixture of both increased proximately 7 %,9 % and 2 % respectively, the difference was significant(p<0.01). The degradation rate of NDF in sheep rumen increased 2～3% and even reached to almost 5% in low-moisture alfalfa silage, the differences were significant(p<0.01), and the interaction among Lactobacillus casei, Lactobacillus maize, Lactobacillus Jensenii, Lactobacillus kimchi in treatment group 5 were the best .