Effects On Mixed Additive Of Lactobacillus And Cellulose Decomposition Bacteria On The Silage Quality

With the development of animal husbandry, silage has been paying attention theworld. In the silage, use the lactic acid fermentation can create an acidic environmentand inhibit the growth of harmful microorganisms, improve silage fermentationquality, nutritional value. However, due to the high content of forage cell wallcellulose, cannot effectively improve silage digestibility. A recent study concludedthat use of cellulase enzymes can effectively solve the crude fiber digestibility andincreased content of fermentable sugars. However, the higher cost of the enzymepreparation, preparation complicated. The main ourpose of this research is the use oflactobacillus and cellulose-decomposing bacteria(isolated by research group)developed a low-cost, high-efficiency hybrid bacteria additives in order to establish ahigh-quality feed production models.1First, will two lactobacillus(Lactobacillus plantarum and Leuconostoc dextransubspecies) and two cellulose-decomposing bacteria(both of Stenotrophomonasmaltophilia) by mixing equal proportions of different ways to add to a2%sucrosesolution culture, determination and draw its growth curve and stable period viable cellcount. Experimental results show that each experimental group to achieve stablegrowth period, when lactic acid bacteria and cellulose-decomposing bacteria toincrease the proportion and number of viable cells, the preliminary judging these twobacteria can no mutual inhibition. The experimental groups of four strains are mixedto stabilize the joint shortest period of time (16h) and higher than the total number ofviable cells in other groups, can be used as silage additives after study section.2performed with fresh feed corn stalks and sorghum straw silage fermentationexperiments laboratory bottles, mixed in different proportions strains as microbialadditives, determined its chemical composition, organic content, dry matterdigestibility of neutral detergent fiber, etc., and have achieved good silagefermentation quality has improved significantly. The results show that thecharacterization of corn silage, forage efficient mixing lactic acid bacteria andcellulose-decomposing bacteria can be added to reduce feed silage pH of3.8or less. Each treatment group lactic acid, acetic acid content was significantly higher (p<0.05), lactic acid content than the control group, the average has doubled. Crudeprotein (CP) content was significantly higher than control group (p <0.05), dry mattercontent (DM), neutral detergent fiber (NDF), acid detergent lignin (ADL), celluloseand hemicellulose content decreased significantly (p <0.05), DM, NDF digestibilitywas significantly higher. Can determine the lactobacillus, cellulose-decomposingbacteria added to the mix of corn stover can significantly improve silage fermentationquality and nutritional value.3stalks of sorghum silage experimental results show that the sensory evaluationbelong high quality feed, additives can be mixed bacterial elevated lactic acid levelssilage; significantly reduce feed pH value, the minimum fell to3.89, the crude proteincontent increased significantly improve the good fermentation quality. Feed aciddetergent lignin and cellulose content was significantly reduced(p <0.05), NDF, ADFcontent also declined, but not obvious. DM and NDF digestibility was significantlyhigher than the control group（p<0.05）.4stalks of corn silage mix strains best addition amount1%:2%,2%:2%,2%:3%;sorghum silage lactic acid, cellulose-decomposing bacteria optimal addition amount1%:3%,2%:2%. This result provides a basis for the establishment of high-qualityforage production models.