Study On The Sucrose Metabolism In Embryogenic Callus Of Occurrence Process Of Picea Mongolica

The Picea mongolica was the unique conifer species in Ba Yin Ao Bao Nature Reserve of Inner Mongolia, which have a higher utilization of the value of tree species to explore in vitro propagation techniques, the establishment of rapid propagation system for the genetic improvement of the species bio-engineering research and development work to lay a good foundation for the theory and technology. In this paper, t the explants was he mature embryos of Picea mongolica ,have searched the sterilization time, different concentrations of disinfection on the survival rate of mature embryos, and to further explore the embryogenic callus of Picea mongolica pathways and the process of callus of sucrose and sucrose content of the changes of enzyme activity.The results were as follows:1.The best disinfection program of explants of Picea mongolica was based on the 70% alcohol to deal with post 30s, with 10% sodium hypochlorite to deal with 10min. This have the lowest rate of pollution, and the highest rate of survival, which played a satisfactory sterilizing effect.2.The best hormone combination of callus induction of Picea mongolica was 0.5mg/L6-BA, 5.0mg/L2,4-D; the best training proliferation hormone combination was 1.0mg/L6-BA, 0.5mg/L2,4-D; differentiation medium of callus of Picea mongolica was LS +20g/L sucrose +3mg /LABA +50g/LPEG4000.3. In the callus induction phase of Picea mongolica, the proliferation stage and mature stage of training, the sucrose content of callus tissue were cultured at the highest value 6d. Induction phase in which content of 13.90% percent, 5.28% percent of value-added stage, differentiation stage of 6.2%.4. Changes of sugar-converting enzyme. In the embryonic callus induction stage, proliferation and differentiation stages of training phase, acidic sugar-converting enzyme is highest in the first 6d, respectively 52.42U/g, 86.26U/g and 125.65U/g. In the embryogenic callus induction stage, basic transformation of the first 6d enzyme activity is highest for 47.37U/g, proliferation and differentiation stages of the first phase up to the highest 3d, respectively 74.14U/g, 67.07U/g.5.The zygotic embryos of Picea mongolica for explants for inducted callus. In the proliferation phase, the hard green non-embryogenic callus sucrose content, enzyme activity of sucrose were higher than white and filamentous embryogenic callus; In the differentiation mature stage, the green buds at the high incidence of embryogenic callus in sugar content and sucrose enzyme activity were higher than the hard surface of the green non-embryogenic callus.