Studies On The Regeneration System From Sour Jujube Leaves And Adventitious Bud Regeneration From Vitro Non-bud Stem Of Ziziphus Jujuba Mill. 'Fupingdazao'

In this study, in vitro sour jujube leaf material and non-bud stem of Ziziphus jujuba Mill.'Fupingdazao'for the test, the impact of leaves in vitro adventitious shoot regeneration of factors such as medium composition, growth regulator type and concentration ratio, the type of inoculation materials and training methods, such as culture conditions, as well as a systematic study successfully established in vitro sour jujube leaf regeneration system.And the factors affecting adventitious bud regeneration in vitro non-bud stem of Ziziphus jujuba Mill.'Fupingdazao'in MS media were studied, which included sterilization time, dark incubation time and plant growth regulator. The main results were as followed:1.Gaining a high effcient regeneration systen in sour jujube in vitro leaf.(1)As to sieving the different mediums type,MS and WPM was confirmed as the best suitable medium. The applying of 30g/L Glucose as the carbon source was adopted.(2)The different plant growth regulators had significant effects on leaf regeneration. It showed that both of cytokinin 6-BA and TDZ could induce regeneration of in vitro leaves of sour jujube. But the 6-BA induction reproducibility is low extremely,so induces its leaf blade regeneration the cell division element to select TDZ suitably, the optimum concentrations was 0.5mg/L. Lower concentration of auxins could induce it regenerate adventitious buds, the optimum auxins for sour jujube was IBA 0.10mg/L.(3)It was found by studying that the regeneration rate was the highest if the leaves came from the tube-plants being subcultred 10~15 times,the leaf age from 30~40 days had higher regeneration rates. The regeneration adult plant and continues a generation of adult plant the leaf blade regeneration effect non-significance difference.(4) It was found by study photoperiod that dark incubation was necessary for leaf regeneration. The regeneration rate rose obviously after incubation in the dark. Along with the daily illumination time growth, the reproducibility has the drop, the illumination time is longer, injuries the organization to grow many. (5) In medium supplemented with a certain concentration of AgNO3, tryptone, peptone and it found that an additional 1~2 mg/L AgNO3, 500 ~ 1000 mg/L tryptone leaves to enhance the rate of adventitious bud regeneration, peptone can not be the promotion of renewable sour jujube leaf.(6) In this study,the method of two-step culture was applied, first of all, the leaves were vaccinated in the differentiation medium, the dark for 21 days, and then transferred to MS medium induced shoot regeneration, the leaves don't easy to browning for this method, the more elongation of adventitious buds quickly, and without glass, regeneration rate step culture method significantly increased.2.MS+6-BA 0.5mg/L +IBA 0.1mg/L+GA3 0.1 mg/L was suitable for elongation of the plantlets from regeneration.3.MS+6-BA 0.5mg/L+IBA 0.1mg/L was the best proliferation medium for the plantlets from regeneration.4.The plantlets from regeneration of sour jujube should be cultured on 1/2MS +IBA 1.0mg/L+NAA 0.3mg/L for 5 days, The role of auxin into the 7 ~ 10d, and then be transferred to medium without Auxin, the regeneration rate were 98.85%.5.MS+6-BA 0.5mg/L+IBA 0.1 mg/L was the best regeneration medium for non-bud stem from Ziziphus jujuba Mill.'Fupingdazao'.