Detetion Of Hepatitis E Virus In Pig Products From Abattoirs And Establishment Of HEV Infection Model For Rats

Hepatit E virus(HEV) is a zoonotic pathogen and pig is generally considered as a major reservoir. HEV replicates in swine group and has been a potential threat to the public health. This study presents methods of detection for Hepatitis E Virus, investigates the prevalent status of HEV in pig products from several large-scale slaughterhouses, and establishes HEV infection model in SD rats.1. HEV is transmitted through the fecal-oral cycle and causes sporadic and epidemic hepatitis in human beings. Traditionally, the HEV infection is diagnosed mainly by detection of anti-HEV antibodies using the ELISA method. However, many drawbacks of this method make the diagnosis difficult. Our study established two PCR methods for swine HEV RNA detection successfully. According to the published genome sequences of HEV, we designed a set of nested primers and presented a RT-nPCR method for swine HEV detection, which was specific and sensitive and could be used for HEV epidemiologic survey. At the same time, a specific and reliable real-time PCR method was developed in this paper, which can be used for further study.2. The bile, liver, spleen and lymphonodi mesenterici of 120 swine were collected and 126 swine fecal and 123 serum specimens were randomly collected from slaughterhouses. The HEV RNA was detected by nested RT-PCR, the HEV positive PCR products were sequenced and sequence analysis was then performed using bio-softwares. Results indicated that bile and serum were the most sensitive fields to HEV. This suggested that the slaughterhouses should pay more attention to slaughter products in order to avoid HEV contamination.3. In this study, the feces positive for HEV was used as sources of infection to SPF SD rats. Using PCR detection methods, the rat model was characterized. In this paper, a detection method of PCR was established. A mouse model of HEV infection was established. This study provided a theory and technique reserve for HEV epidemic outbreak, and it's significant for formulating the integrate control program of HEV.