| Variety is the most important basis for tea production. They play irreplaceable role in those aspects of increasing tea yield, improving tea product quality, enhancing tea product competition and developing efficient tea industry. A new tea strain, which was named as'Lizaoxiang'subsequently, was discovered in Lishui city Zhejiang province in the collection program of tea germplasm during 2000-2003. This thesis studied the morphological and the biological characteristics as well as the suitability of manufacture of this strain. Meanwhile, the identifying and evaluating works were also done by using the chemical fingerprint techenique, molecular mark techenique and DUS analysis. The results were as follows:'Lizaoxiang'is shrub form, small to middle leaf, semi-erect type. The angle of leafing is horizontal with a little upright. The leaf shape is oblong oval and the color of mature leaf is light green. Leaves extend plat with a little upheaval and a little inward-folding. Leaf apex is blunt and then taper-pointed. Leaf marging is a little zigzag. And bud and leaves are with numerous hairs. The average number of calyx is 6.2 and is puple with no hairs. Corolla is white and its diameter is 3.7cm. The average number of petal is 6.5 and the length of style is 1.4cm. Ovary has dense hairs and the ratio of pistil to stamen is high. Full flowering period is in the middle of October and lasts for about one and half month but consequently gives scarce seeds.'Lizaoxiag'is extra-early sprouting and its date of one bud and a leaf is 1-3 days earlier than that of extra-early sprouting variety'Wuniuzao', 7-10 days earlier than that of'Longjing 43'. It has dense shoot density and its one-hundred-bud weight is a little higher than that of'Longjing 43'. It has good leaf tender keeping ability and good shoot vertical. The yield of fresh leaf is 15.1%-28% higher than'Wuniuzao'. Also, its cold resistence is better than'Wuniuzao'but the ability of anti tea pink rust mite is not very good. The level of amino acid in processed tea made of one bud and two leaves is 4.3%, that of tea polyphenol is 31.36%, caffeine 3.13% and water extract 47.6% with a polyphenol to amino acid ratio of 6.82. The leaves plucked before the third to the fifth shoot vertical of spring tea are much stout and suitable for processing flat-shaped green tea, those plucked in the middle stage suitable for spiral green tea. The quality of these tea products is superior according to the result of testing made by the Tea quality supervision and testing center of Ministy of Agriculture.The composition of tea catechin of'Lizaoxiang'is similar to that of'Longjing 43'according to the result of chemical fingerprint study, but'Lizaoxiang'was regarded as more similar to those jats like'jiukeng'based on the similarity of overall chemical fingerprint. On the model diagram of chemical fingerprints, the differences of composition or ratio of main biochemical componants of'Lizaoxiag'to other varieties for contrast could be seen clearly and obviously. And those products made by'Lizaoxiang'variety could be identified from those of other three contrast vatieties by the method of stepwise discriminant analysis.The study using inter-simple sequence repeat (ISSR) marker techinique revealed that'Lizaoxiang'could be identified from other four contrast varieties by using four primers selected from total 10 ones. The results of genetic distance (GD), genetic identification (GI) and similarity coefficient analysis as well as that of cluster analysis based on ISSR markers were all consistent, which indicated that'Lizaoxiang'had closest genetic relationship with'Wuniuzao'and farest one with'Longjing 43'in those varieties tested. |
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