| Venison is famous for its rich in protein, lower in fat, easy digestable, and very nutritious, tender and nicetaste. Therefore, it is very popular as it can enhance human body metabolism and strengthen resistibility to disease. In the present study, I chose the MyoG gene as the candidate gene that can influence meat production in deer, based on the findings of other species(cow, goat, pig, human, mouse, )MyoG gene, the MyoG gene of Gansu wapiti was cloned by PCR. The characteristics and the molecular structure of the MyoG gene were analyzed and predicted by the bioinformatics. Then the polymorphisms of the MyoG gene 5'UTR in Gansu Wapiti were analyzed by PCR-SSCP. The PCR products of different genetype were cloned and sequenced. Then I analyzed the sequence and some transcription factor binding sites were predicted after mutation. The main results were as follows:(1)The full length of myogenin(MyoG)gene of Gansu Wapiti was firstly cloned and sequenced. Its DNA sequence was 3168 bp in length, including three exons and two introns. The length of 5'UTR and 3'UTR sequence was 381bp,696bp respectively. The lengths of three exons which encoded 227 amino acid were 480bp, 82bp and 122bp respectively. The lengths of two introns were 799 bp and 608bp respectively. Introns and exons were separated by GT/AG, which is consistent with the character of eukaryote genetic DNA. The sequence has been submitted to GenBank and the accession number is FJ746497.(2)Analysis of nucleotide composition of the gene showed that G/C contents(57.8%) were abundant, and the G/C contents(62.7%) in exon regions were significantly higher than in intron regions(55.5%). The nucleotide homology of Gansu Wapiti MyoG gene with cattle, goat, horse, pig, house mouse, Norway rat, human, chicken, turkey, zebrafish, catfish, African clawed frog, western clawed frog was 95.7,96.6,89.6,90.9,87.5,87.2,88.0,70.5,68.5,55.5,56.1,54.5 and 55.3% respectively. The amino acid homology of Gansu wapiti MyoG with cattle, goat, pig, house mouse and human was 98.7,98.7,96.4,93.8和93.3% respectively.(3)The predicted results of physical and chemical character, secondary structure and conserved functional domain of MyoG protein were indicated that: the molecular weight was 25kDa, theoretical isoelectric point was 5.68, secondary structure were mainly theα-helix and random coil. There is a basic-helix-loop-helix domain from 1st to 136th amino acid of Gansu Wapiti MyoG protein which shared with same domain with other members of MRFs.(4)The molecular evolution trees of MyoG gene showed that the distance between Gansu wapiti, goat, cattle, pig and human were the nearest, between birds was nearer and between fish was the most far. Based on animal taxonomy, Gansu Wapiti, goat and cattle belong to ruminant. Therefore, the molecular evolution tree of MyoG matches that from animal taxonomy.(5)The results of PCR-SSCP analysis indicated that there is polymorphism which was controlled by two alleles: A and B in 5'UTR of Gansu Wapiti MyoG gene. The mutation of G→A at -237 converted allele A into B. A is the dominant Allele gene in Gansu Wapiti. They were in low polymorphism in PIC mutation site in Gansu Wapiti by other analysis. There would be one Sp1 transcription factor missing at the mutation site after transcription factors binding site prediction.
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