Effect Of Bioactivities And Crop Safety Of Emamectin Benzoate Substituted Plant Oil For Xylene

New requirements of green pesticide are proposed for better pesticide solvents recently. Plant oils, which are recycle resources that environmentally friendly, high biodegradation rate, compatible to target, are the first choice to replace organic solvents. Using three kinds of plant oils, including refining pine oil with palm, refining pine oil, and palm oil, we studied the effect of bioactivities and crop safety of 1% emamectin benzoate substituted different plant oils for xylene, such as bioactivities and enzymatic activities of Plutella xylostella L., and physiology and biochemistry of Brassica campestris L. The concrete results are as follows:Allied toxicity measurement showed that two kinds of plant oil solvents combinations increased the insecticidal activity of emamectin benzoate EC significantly. The toxicity index were 0.709 and 0.566 respectively, had synergism. However, as a solvent, palm oil decreased the insecticidal activity of emamectin benzoate EC, its toxicity index was 4.848, decreasing the effect of pesticide.The respiration rate of Plutella xylostella L. showed that the emamectin benzoate EC dissolved in plant oils inhibited the respiration rate, furthermore, the quantity of CO2 that treated with high concentration emamectin benzoate EC which dissolved in plant oils was less than that dissolved in xylene.After treatment with emamectin benzoate EC substituded plant oils for xylene, the activities of digestive enzymes of Plutella xylostella L. showed that the AMS increased, while the pepsin and LPS decreased. The activities of protective enzymes showed that the POD specific activity was lower than that dissolved in xylene, the SOD and CAT specific activities decreased at LC20 but increased at LC50. The activities of detoxifying enzymes showed that the GSTs specific activity increased at LC20 but decreased at LC50. And the AChE specific activity was inhibited significantly.Emamectin benzoate EC dissolved in xylene and palm oil leaded to phytotoxicity to brassica campestris L.but the others not. The new leaves that applied emamectin benzoate EC dissolved in xylene were malformed and not recovered. Another dissolved in palm oil caused the young leaves withering and wilting, but the symptoms disappeared with the leaves growth.After applied with emamectin benzoate EC dissolved in plant oils, nutrients of Brassica campestris L. showed that the protein content decreased, and the amino acid had a small increase, but no effect to chlorophyll. The height of Brassica campestris L. showed that there are no effects to its growth.The research results showed that two kinds of plant oil solvents combinations increased the insecticidal activity of 1% emamectin benzoate EC significantly,and safety to Brassica campestris L. They had great potential on the production.