Using The Microarray Technology To Study On Differential Expression Gene Of Alhagi Sparsifolia Root Under Salinity Stress

In this research,we serve Xinjiang halophyte leguminous plants Alhagi sparsifolia as plant material.The effect of different concentration NaCl on the germination of Alhagi sparsifolia seed was studied.In order to understand differential expression gene in Alhag sparsifolia root,the research was studied by Medicago Genome Array(61278 probes) of Affymetric company.The root had been exposed to 220 mmol/L NaCl for Oh and 24h.By GO annotation analyzing these differential expression genes.Designed the primer combining PCR technology to clone the function gene of the probe in Alhagi sparsifolia The in vitro propagation of Alhagi sparsifolia by cuttings was established as the base of seedling salt tolerance validated.The results as follow:1.The Alhagi sparsifolia seed germination rate was 100%when the NaCl solution below 300mmol/L.It still germinated in 700mmol/L and the seed germination rate was 40%. Between the 400-700mmol/L,the seed germination rate was decline as the NaCl concentration increasing.The root length was negative to the NaCl concentration.2.In the 61278 probes,5133 genes were screened in Oh root and 5378 genes were screened in 24 h.There were 246 differential expression genes,the up-regulated gene was 147 and down-regulated gene was 119.3.By GO annotation analyzing parts of differential expression gene,these differential expression genes main related to energy metabolism,signal transduction,transport and cell wall synthesis.4.The FDH gene in Alhagi sparsifolia was cloned by PCR,got the codon sequence 840bp,which encoding 280 amino acid.The codon sequence and amino acid sequence of the FDH gene was shared by 91%and 90%identity with the same gene family Lotus corniculatus FDH gene respectively.5.The better induction medium of Alhagi sparsifolia was MS+6-BA 0.5mg/L+NAA 0.1mg/L+LH 1.5g/L,the propagation index of adventitious bud was 6.8.LH was advantaged to seedling growth and differentiation of the bud.The effect of GA₃ on in vitro propagation of Alhagi sparsifolia was not significant.