Effect Of Supplementing Sodium Diacetate To Baled Total Mixed Ration On Storage And Performance Of Dairy Cows

Two experiments were conducted to investigate effects of different levels of sodium diacetate (SDA) on storage and performance for dairy cows.ExperimentⅠ: Effect of the different levels of sodium diacetate supplementation on the storage of the baled total mixed ration (BTMR).This trial was designed to study the storage effect of different levels of SDA on storage of the total mixed ration BTMR of forage to concentrate ratios 50:50 and moisture content 50%. The SDA supplements were 0, 0.1%, 0.3%, and 0.5% of the fresh weight of TMR. After 0, 3, 7, 15, and 30 days storage, the sensory was evaluated and the chemical composition, fermentation characteristics, and aerobic stability were determined. Each treatment had three repetitions. The results indicated all treatements kept well after 15 days storage, but the treatments of control and 0.1%SDA were Moldy after 30 days storage, and the treatments of 0.3% and 0.5% SDA kept well. The baled TMR with higher SDA level had the higher aerobic stability. The effects of different levels SDA supplement on the chemical compositions were not different. The baled TMR of 0.3% and 0.5% SDA addition had lower pH (P<0.05) and NH3-N/TN (P<0.05) compared with other groups. The lactic and acetic acid content increased at the period of storage time, and the Propionic acid was no difference at the storage. Butyric acid could be determined in the control and 0.1% SDA at the beginning and end of the storage. Considering the economic benefit and addition effects, it was suggested that the optimal level of SDA supplement was 0.3%.ExperimentⅠ: Effect of baled total mixed ration (BTMR) supplemented with sodium diacetate on performance and digestive characteristics of dairy cowsThis experiment was conducted with thirty Holstein cows in mid-lactation (124±23 DIM, 17.29±7.29 kg of milk/d ) to examine the effects of supplementing sodium diacetate in baled total mixed ration (BTMR) on dry matter intake (DMI), milk yield, milk composition, body condition score (BCS), and some serum biochemistry parameters. Treatments were: 1) the forage and concentrate feeding separately (FS); 2) feeding in the form of baled total mixed rations(BTMR); 3) BTMR supplemented with sodium diacetate (SDA). Animals were housed and individually fed 3 times daily at 0230, 0830 and 1430 h to allow 5 to 10% orts (as-fed basis) in a tie-stall barn and had continuous access to water. Feeding intake and orts were recorded daily. Cows were milked twice daily at 0200 and 1400 h. Blood samples were collected monthly via venipuncture from coccygeal vein 3 h after morning feeding. The data were analyzed using the MIXED model of SAS 9.0 with a repeated measure. The results indicated that the DMI of the BTMR and SDA were significantly (P<0.01) higher than FS (18.9, 18.7 and 17.7 kg/d). The treatments of BTMR and SDA increased milk yield by 6.2% and 7.2% compared with FS. The milk protein, milk lactose, and solid non-fat (SNF) showed no significant differences among three treatments. But the SDA treatment significantly (P<0.01) increased the milk fat compared with BTMR and FS (3.86, 3.27 and 3.4%). There were 25.11% and 17.28% reduction in SCC for SDA group compared with the BTMR and FS groups. The BCS values of cows fed BTMR, SDA and FS diets were not significantly different. Blood urea nitrogen(BUN) concentrations of the BTMR and SDA groups were significantly (P<0.01) lower than that of FS group (21.8, 20.9 and 26.4 mg/dL), and no significant differences existed among three treatments on serum protein, serum glucose and serum lipid concentrations. The SDA and BTMR group apparent digestibility of crude protein and fat were significant higher than that FS group (P<0.01), while no difference was observed in that of other nutrients among three groups (P>0.05).These results suggested that BTMR and SDA had positive effect on feed intake and milk production of lactating dairy cows compared with FS, while the addition of SDA increased milk fat significantly.