Investigation On The Antibiotic Resistance And The Role Of Class Ⅰ Integron Gene Of The Pathogenic Isolates Of E. Coli From Poultry

In recent years, many research and reports on the resistance of E. coli-related have been published, especially in the type I integron and integrase gene detection research. 37 pathological isolates of E. coli were selected for drug resistance tests. The antibiotic resistance and ESBLs of 37 isolates were tested by KB method and automatic analyzer of bacteria. The total DNA I integration on IntI 1 and IntI1I-positive specimens of Class I Integron (IntI) were amplified by PCR method. Int integron was analysed by the enzyme cuts, and Class I Integron purified was conducted. The results of DNA sequencing were compared with the DNA sequence in the Gene-Bank so as to confirm the type of integration of variable-box gene type and ranking.The results showed that E. coli resistance to antibiotics is serious, the strains isolated in recent years have the higher resistence rate than that in the earlier year, and the more early antibiotics were used in practice, more the higher resistence rate is.The I-type integrase gene and integration from E. coli isolated were successfully amplificated by PCR method. The results showed that 30 isolates among 37 E. coli isolated were tested positive for E.coli IntI1, and 23 isolates were amplified IntI among the 30 isolates of positive for E.coli IntI1. The results also showed that multi-drug resistant often exit in isolates positive for E. coli IntI1, and there is the close relation between both.7 kind of Integron genes were Altogether detested, and the size is mainly between 500bp~3300bp. The most commonly Integron gene examined is the Box-aadA5 (an streptomycin resistant gene), next is the Box-dfrl7 (Sulfadiazine resistance gene). And the size of the same type of integration I that have a same restriction map showed that they may have the same sequence.