Isolation And Purification Of Flavonoids And Antimicrobial Activities From Leaves Of Capparis Spinosa L. And Analysis Of Hemical Components Of Vilatilo Oils

In this study, flavonoids from leaves of Capparis spinosa L. were firstly extracted by conventional extraction and such newly emerging methods as microwave-assisted extraction (MAE) and ultrasonic-assisted extraction. Through comparision of different methods and experimental designs as well as analyses of experimental data,an optimal condition for extraction was worked out.Then the flavonoid extracts were further separated and analyzed. Several method have been compared to extract the volatile oil from leaves of Capparis spinosa L..The best one is determined by volatile oil detector.The components of volatile oil extracted from leaves of Capparis spinosa L.were identified by GC-MS.1. Flavonoids from leaves of Capparis spinosa L. were extracted with the ultrasonic-assisted extraction (UAE), and the antibacterial function of different components extracted by ethanol was discussed. The results showed that the optimum extraction is as follows:adding 75% ethanol into leaves of Capparis spinosa L.,extracting 50min;the rate of solid and liquid is 1:25; and number of extraction cycles is 5. Both ethyl acetate fraction and n-butanol fraction of the species had stronger antimicrobial activity than that of petroleum ether fraction.2. To investigate the technical parameters of the purification process of total flavonoids of leaves of Capparis spinosa L. with AB-8 macroreticular resin, the parameters were optimized. Response surface methodology (RSM) was employed in the study and a mathematical regression model for purification of flavonoids from leaves of Capparis spinosa L. with the macroporous resin was built. Results showed that the optimal adsorption parameters are adsorptive velocity 1.5mL·min-1,pH 3.3,solution concentration 1mg·mL-1 and the optimal desorbtion parameters are 70.00%ethanol as eluting solvent,pH 6.7,the ethanol volume 6BV.3. The essential oil in the leaves of Capparis spinosa L.was extracted by steam distillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Fifty chromatographic peaks were isolated and thirty-four compounds were identified,accounting for 94.93% of the total volatile oil in leaves of Capparis spinosa L..