Detection Of Single Nucleotide Polymorphisms (SNP) For Amylose Extender Gene (ae) And Content Determination Of Amylose In Backcross Progeny

Amylose is one of the most important raw materials for industrial applications. It has been widely used in a variety of fields, such as light industry, food industry, pharmaceutical industry, production of films. It can also be used to produce degradable plastics so that the environmental pollution can be fundamentally solved. Therefore, the study on gene ae has great significance on cultivating and developing exclusively used new variety of maize with high quality and high yield.Using four high amylose maize inbred lines and six normal inbred lines as materials, the paper studies the differences among sequence of ae gene of different maize, which established a good foundation to mark gene ae and shorten the period of breeding in the future. In order to select the superior ae gene donor parents and recurrent parents, donor parents of three ae genes and eight maize inbred lines were used to cross each other. The seeds of inbred lines and BC₁F₂ or BC₂F₂ of 24 cross were used to test the amylose content. the main results are as follows:1. According to the sequence of gene ae reported in Genbank, 19 pairs specific primers were designed and carried out PCR above materials. PCR production were separated and the sequence were measured. The results show that only one deletion with three bases(CTT)at locus between 10546 and 10547; one insert with one bases (C) at locus 10597 was found, two transitions with one base (C→T) at locus 10380 and (T→C) at locus 10410. one transversion with one base (T→A) at locus 18137.2. Donor parents of three ae genes and eight maize inbred lines were used to cross each other. The seeds of inbred lines and BC₁F₂,BC₂F₂ of 24 cross were used to test the amylose content. The results showed that compared to maize inbred lines, the amylose content increased in BC₁F₂,BC₂F₂ of most cross. Expression capacity of different donor parents of ae genes were significantly different in backcross parents that was under different genetic background. The superior ae gene donor parent is W23aeae. The superior recurrent parents are K12 and zong3.