The Heterosis Research Of Rice Nitrogen Metabolism Enzyme Activity

Ganghui128, Changlunzhen, Aixiuzheng, BG367, 6827 and Qiguizhao and their complete double row hybrid generation were used as explants. And nitric acid reductases (NR) and the valley ammonia amide synzyme (GS) activeness under the condition of different period of birth duration, the different nitrogen density induces of them were studied. The results show:1.Ganghui128,Changlunzhen,Aixiuzheng,BG367,6827,Qiguizhao, 6 different genotype rice species, in four different period of growth stages (seedling time, tillering stage, earing period, mature period) , the valley ammonia amide synthesis enzyme activity displays certain change rule under the different nitrogen density condition induction, Each species in every stage of growth, the valley ammonia amide synthesis enzyme activity displays highest in various stages with the nitrogen density 40% (nitrogen content of total minerals nutrition proportion) induction,the valley ammonia amide synthesis enzyme activity displays that the first increase then induce change along with nitrogen density increase in identical period of growth stage ; Similarly, in the identical period of growth stage, the nitric acid return to original state enzyme activity displays certain change rule under the different nitrogen density condition induction, Each rice species at the same stage of growth , with the nitrogen nutrition density 60% induction, the nitric acid return to original state enzyme activity displays highest, but the nitric acid return to original state enzyme activity increases along with the nitrogen density inceasing in each period of growth stage; the nitric acid return to original state enzyme activity change tendency obviously,however, is differen in different nitrogen density scope, the nitric acid return to original state enzyme activity enhancement speed is biggest with 20%--40% desity of nitrogen condition induction, the active enhancement speed is smallest under 40%-60% desity of nitrogen condition induces.The nitrogen nutrition density is an important external condition which induces the rice body activity of nitric acid return to original state enzyme and the valley ammonia amide synthesis enzyme; Therefore, the suitable nitrogen nutrition density is one of essential condition of maintains the rice high nitrogen nutritional absorption and utilization efficiency.2. Ganghui128,Changlunzhen,Aixiuzheng,BG367,6827,Qiguizhao 6 different genotype species, in four different nitrogen densities (0%, 20%, 40%, 60%) condition induction, the nitric acid return to original state enzyme activity and the valley ammonia amide synthesis enzyme activity displays certain change rule along with the different period of growth stage,, the nitric acid return to original state enzyme activity and the valley ammonia amide synthesis enzyme activity of each species displays the highest time is the tillering stage under the identical nitrogen density condition induction, activeness displays the lowest time is a mature period; under the identical density nitrogen nutritional conditions induction, the nitric acid return to original state enzyme activity and the valley ammonia amide synthesis enzyme activity show that the first strengthens the weak trendency along with the period of growth change.3. Ganghui128,Changlunzhen,Aixiuzheng,BG367,6827,Qiguizhao 6 different genotype rice species as well as its 30 complete double row hybridizes generations, its hybrid generation's nitric acid return to original state enzyme activity and the valley ammonia amide synthesis enzyme activity displays the similar regularity in the tillering stage and the earing period, the hybrid generation the nitric acid return to original state enzyme activity and the valley ammonia amide synthesis enzyme activity are higher than its parent in the identical period of growth, and the female parent activeness performance is higher, enzyme activity aspect of hybrid generation displays more prominent, but the influce of male parent is not big to the hybrid generation's.