The Protection Effect Of Vitamin E On Reproductive Performance And Immune Function In Female Mice Infected With Pseudorabies Virus

The study was conducted with the objective of investigate the effect of vitamin E(VE) level on reproductive performance and immtme function of 9th pregnant mice infected with Pseudorabies Virus.152 six-week-old Kun-ming SPF female mice fed with VE deficient dietary.After the model of vitamin E deficiency was established,the mice were randomly divided into 8 groups.Mice in the five experimental groups were infected with Pseudorabies Virus and supplemented with Vitamin E at 0,75,375,750 and 1 500 mg/kg, respectively;mice in the three control groups were injected with PBS and supplemented with vitamin E at 0,75 and 375 mg/kg,respectively.Mice were fed experimental diet for four weeks and mated with male mice.Female mice were injected with Pseudorabies Virus(SA215 strain) or PBS on 0^th pregnancy.Mice were killed on 9th pregnancy and collected serum,brain,liver,uterus and embryo.The hepaticα-tocopherol concentration, serum immunoglobulin(IgG,IgA,IgM) concentration,IL-2,IFN-γ,IL-10 concentration and the and the mRNA expression of the toil-like receptor 3,7 and 9(TLR3,TLR7 TLR9) in the uterus and embryos were determined.Every group left 5 mice to delivery and recorded the live litter size.Results show that:1.The content of hepaticα-tocopherol of challenged mice was increased with vitamin E level raised.It was significantly lower in 0 mg/kg group than that in other groups(P＜0.05).On same level of VE,it was lower in challenged groups than that in control groups,besides the difference was significantly(P＜0.05) on the level of 75 mg/kg VE.2.The mortality and abortion rate were decreased with vitamin E level raised in challenged groups.The abortion rate was significantly lower in 1 500 mg/kg group than that in 0 mg/kg group(P＜0.05).3.The live embryos number on 9^th pregnancy of challenged mice was significantly lower in 0 mg/kg group than that in other groups(P＜0.05),and there was no significantly different between the other groups(P＞0.05).The live embryos number of challenged mice was increased quadratically with VE level raised,it reached the maximum on the level of 987 mg/kg VE.The live litter size was significantly higher in 375 mg/kg group than that in 0 mg/kg group(P＜0.05).There was no significantly different between 0 mg/kg group and 1500mg/kg group.The live litter size of challenged mice was increased quadratically with VE level raised,it reached the maximum on the level of 790 mg/kg VE.Also,on same level of vitamin E,the live embryos on 9^th pregnancy and live litter size were lower in challenged groups than in control groups,especially on the level of 75 mg/kg VE,the live embryos was significantly lower in challenged group than in control group(P＜0.05).4.In the challenged groups,the spleen index was significantly higher in 750 mg/kg group than that in 0 mg/kg group and 75 mg/kg group(P＜0.05) and higher than 1500 mg/kg group(P＞0.05).It was higher in challenged groups than that in control groups(P＞0.05) on same level of VE.5.The serum concentration of IgG on 9^th pregnancy of challenged mice in 0 mg/kg group was significantly lower than that in 375 mg/kg group and 750 mg/kg group(P＜0.05). There was no significantly different between 750 mg/kg group and 375 mg/kg group(P＞0.05),and they were significantly higher than 1500 mg/kg group(P＜0.05).The serum concentration of IgA in 375 mg/kg group was significantly higher than that 0 mg/kg group,750 mg/kg group and 1500 mg/kg group(P＜0.05).The serum concentration of IgM in 375 mg/kg group was significantly higher than that in other groups(P＜0.05).On same level of VE,the serum concentration of IgG,IgA and IgM in challenged groups were higher than that in control groups.And on the level of 375 mg/kg VE,the serum concentration of IgG and IgM in challenged group was significantly higher than that in control group(P＜0.05).6.The serum concentration of IL-2 and IFN-γof challenged mice were decreased with vitamin E level raised.They were higher in 0 mg/kg group than that in 375 mg/kg group,750 mg/kg group and 1500 mg/kg group(P＜0.05 or P＞0.05).The serum concentration of IL-10 in 0 mg/kg group was significantly lower than that in 375 mg/kg group(P＜0.05),and it was significantly higher in 375 mg/kg group than that in 1500 mg/kg group(P＜0.05).On same level of VE,the serum concentration of IE-2 in challenged groups were significantly higher than that in control groups,the serum concentration of IL-10 in challenged groups were lower than that in control groups.And on the level of 0 mg/kg and 75 mg/kg VE,the serum concentration of IFN-γin challenged group were significantly higher than that in control group(P＜0.05). 7.The expression of TLR3,TLR7 and TLR9 in uterus of challenged mice in 0mg/kg group were significantly higher than that in 375 mg/kg group,750 mg/kg group and 1500 mg/kg group(P＜0.05).On same level of VE,the expression of TLR3 and TLR9 in uterus of challenged groups were higher than that in control groups(P＜0.05).And on the level of 0 mg/kg and 75 mg/kg VE,the expression of TLR7 in uterus of challenged group was significantly higher than that in control group(P＜0.05).The expression of TLR3,TLR7 and TLR9 in embryos of challenged mice in 0mg/kg group were very low.It was significantly higher in 75 mg/kg group and 375 mg/kg group than that in other groups(P＜0.05).On same level of VE,the expression of TLR3,TLR7 and TLR9 of embryos in challenged groups were higher than that in control groups. Especially on the level of 75 mg/kg and 375 mg/kg vitamin E,they were significantly higher in challenged groups than in control groups(P＜0.05).Conclusion:1.Marginally deficient in VE,the reproductive performance and immune function of female mice were decrease.Virus infection lead to the mortality and abortion rate significantly increased and the live embryos number on 9th pregnancy and live litter size are significantly decreased.2.Properly increasing dietary VE level can decrease the mortality and abortion rate, and increase the live embryos and live litter size of infected pregnant mice.Clinically infected mice had a higher vitamin E requirement than normal mice.Particularly,vitamin E requirement of female mice with the best reproductive performance could be 10-fold higher than that of normal mice.3.Properly increasing dietary VE level improve immune protection on infected pregnant mice,and increase the level o fhumoral immune.4.Properly increasing dietary VE level can down-regulate expression of TLR3,TLR7 and TLR9 in uterus and embryos of infected mice,and reduce the level of cell immune. 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