Research The Temporal And Spatial Distribution Of Inoculum In High-Temperature Composting Period

This test to cow dung and straw for compost materials, set up three different compost, were added to cellulose-decomposing bacteria (Bacillus) composting; added cellulose decomposing bacteria (Bacillus) and the nitrogen-fixing bacteria agent (Rhizobium is) composting; and control of natural compost. At different times and different spatial sampling, of natural compost and artificial inoculation of the composting process agents the traditional culture method and PCR-DGGE analysis of technology, comparison of artificial inoculation and natural composting in high-temperature phase difference between the diversity of microbial flora. And from different sampling time and sample different aspects of spatial analysis of the two inoculum throughout the composting process and the dynamic changes in the distribution.Analysis of the traditional culture of microorganisms in the composting process studies show that the whole composting process of the greatest quantity of bacteria, fungi, actinomycetes relatively small quantity. Change the number of bacteria was higher - lower - higher - to reduce the trend of the compost inoculum of bacteria higher than the quantity of natural compost. Change the number of actinomycetes were higher in general - to reduce the trend of the two types of compost inoculum in the same period, the number of actinomycetes were higher than the non-inoculated natural composting agents. Throughout the composting process, the quantity changes of fungi was reduced after the first increase in the trend. Inoculum of two lower than the quantity of natural compost compost fungi.Cellulose bacteria inoculated compost, cellulose strip strain at high temperature composting period of widespread distribution in space on the main focus at the middle and upper, middle and lower classes because of poor ventilation, anaerobic fermentation will be conducted, so no agents. Cellulose and nitrogen-fixing bacteria inoculated compost, nitrogen-fixing bacteria present in the main strip of high temperature phase of composting late on the distribution in space can exist in various layers of compost, cellulose bands in which the bacteria was not obvious.DGGE bands similarity analysis showed that high-temperature phase of composting microorganisms exist changes the structure of ecological communities. Comparison shows the diversity, adding agents to increase the diversity of the micro-ecological communities.On the advantages of composting with different periods of time and sequence-specific band has been Uncultured Kaistia sp. Partial 16S rRNA gene, clone AC03 microbial species, such as 26. Through one of alignment found that the high-temperature composting period included Acinetobacter sp. (Acinetobacter), Arthrobacter sp. (An Arthrobacter), Flavobacteria (Flavobacterium) and some other bacteria, high-temperature phase of composting proved the existence of bacteria.