| The mushroom residue were used as the auxiliary organic materials in the rabbit manure composting experiment, and in which aeration windrow composting process was adopted. Two trial groups(mushroom residue-rabbit manure) and two control group (pure rabbit manure), were set up respectively,composting process of about 51 days. The temperature of piles, moisture content, pH,total organic carbon content, total kjeldahl nitrogen content,C/N,total phosphorus content,total potassium content,seed germination index(GI), general bacterial population and then E.coli population of were studied.Use of the biological technique of Denaturing Gradien Gel Electrophoresis understand the development and variation of the microbial structures during the composting,the relationship between the physical characteristic,chemical parameters and the succession of microbial communities, further studies the mechanism of thermophilic fermentation during rabbit manure composting.The experiment in order to understand the physical characteristic and chemical parameters influencing composting and the auxiliary organic materials selecting provide scientific basis for rabbit manure composting. Explored rabbit manures resources,minimization,using the sound of effective channel and provide theoretical basis for rabbit manure composting treatment on large rabbit farms. The following results have been obtained:1. Rabbit manure and mushroom residue cooperation ratio were 2.6:1, initial moisture content of piles was maintained to 55%-60% during the composting, compost pile temperature arrived at the normal fermentation temperature in composting process athe results taken as reference in the actual production.2. Test groups and control groups total organic canbon and C:N ratio were showed downtrend, total kjeldahl nitrogen relative content was increased, total phosphorus and total potassium was maintained to unchangeableness throughout the experiment,similar trend was observed with respect to other domestic animal manure. Test groups the decrease general bacterial population in the process while the decrease was not obvious in control groups.the E.coli were mass mortality in the high temperature period,bacterial reproduction in the cooling-maturity period.3. Total organic carbon,pH,C:N ratio and seed germination index can applied to maturity evaluation of composting productions well.Test groups and control groups,pH were 8.70 and 8.73,C:N ratio were 22.53 and 17.86,the seed germination index above to 0.8, all parameters which indicate the compost was complete mature.4. PCR-DGGE was used to analyzed the structure of the microbial communities. The result showed that the successional of microbial communities of the compost was obviously during the composting. Denaturing gradien gel electrophoresis patterns showed that:the composting process in the quantities of becateria change in ghe ternd was "higher-lower-constancy",in the high temperature period the quantity of microbial was more.5. In the whole composting process, quantity of microbial became more and more,this result accrod with the results by conventional culture. The average shannon-weaver index of bacteria in control groups were higher than test groups (1.82 versus 1.72 to bacteria diversity),showed that bacterial communities in the control group more than in the test groups, but the dominant bacteria were significantly better than test groups. The control groups and the test groups similarity coefficient were above 50% due to the piles microbial from rabbit manure indigenous microbial The bacterial number change tendency was similar to the trend of temperature and opposite tend with the C:N ratio.6. In addition,the change of pH in control group and test groups quite different, other parameters were in line with test group. Control group duration of 50℃high temperature period 9 days, E.coli and GI is also consistent with the requirements of compost maturity, total kjeldahl nitrogen and total phosphorus slightly higher than the test group.The results also showed that the raw rabbit manure could be directly used in composting after moisture content was adjusted to a proper range, and the process was not only simple but also low cost.
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