Effect Of Inoculating Mycorrhizal And Rhizobium On Nitrogen Transferred In Soybean/maize Intercropping System

Intercropping system is an important component of the multiple cropping, it is playing an important role in Chinese agriculture .Intercropping has it the characteristics of full use resources and increase production,so it has practical significance in solve the problem of growing population,resources depletion and environmental degradation, it becomes an important way of the sustainable development of the world agriculture. This experiment is setting different levels of nitrogen,inoculating different mycorrhizal and Rhizobium. Chose the mycorrhizal G.m and Rhizobium SH212.Using the three root partition method,petiole injection 15N tracing technique, researching the effect of inoculating mycorrhizal and Rhizobium on biomass and nitrogen transferred in soybean/maize intercropping system. Clear the amount of nitrogen transferred, have the following conclusions:Whether single inoculating mycorrhizal and Rhizobium or double-inoculating treatment, increasing the biomass of soybean and maize, promoting the absorption of nitrogen. Rhizobium SH212 is better than Rhizobium BA207, mycorrhizal G.m is better than mycorrhizal G.i. Both inoculating mycorrhizal G.m and Rhizobium SH212 is the best one. Average 25.4% higher than no inoculating in total biomass.It is increasing the soybean's nodules number,the rate of mycorrhizal Infection. Promoted the ability of nitrogen fixation of soybean, indirectly improve the nitrogen nutrition of maize. Increasing the total nitrogen uptake of soybean and maize.In soybean/maize intercropping system, through the root partition experiment shows that: For the biomass of maize, no barriers>mesh barriers>solid barriers. The biomass of maize in the root no barriers is 10.5times than solid barriers. The concentration of nitrogen shoot of maize in no barriers is 13.8% higher than root solid barriers. The amount of nitrogen uptake in no root barriers is higher than the other root barriers. It is 1.08 times than the root in mesh barriers,and 1.03 times than the root in solid barriers. It shows that the soybean/maize intercropping system has a synergistic role in promoting, and it is increasing the growth of soybean and maize.In soybean/maize intercropping system, through the petiole injection 15N tracing technique, indicates that the soybean transferred nitrogen to maize. Transfer rate at between 5.19%-9.88%.The amount of transfer nitrogen is 8.19g-18.2g.The amount of transfer nitrogen equivalent to the soybean's amount of nitrogen uptake 7.95%-10.7%.It detected in inoculated mycorrhizal and Rhizobium was going to promote the amount of transfer nitrogen. The rate of transfer nitrogen in root mesh barriers is less than roots no barriers. It shows that the interaction between the root system more strongly, the greater nitrogen transferred significantly. With marking 15N on the maize's plants, didn't find the nitrogen transfer from maize to soybean ,it shows that it's a one-way nitrogen transfer in soybean/maize intercropping system.