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Studies On Culture Conditions Of Lactobacillus And Preservation Process For Producing Fermentation Feed

Posted on:2010-05-17Degree:MasterType:Thesis
Country:ChinaCandidate:P H JiaFull Text:PDF
GTID:2143360278975512Subject:Fermentation engineering
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The present paper through to carries on after the biochemistry appraisal confirmation for pentose piece coccus Pediococcus pentosaceus and the plant lactobacillus Lactobacillus plantarum experimental strain obtains the biggest biomass the most suitable raise condition and mold mushroom spawn's diaper and the freeze-drying protection craft research, causes it to use in producing the micro ecology fermented feed the special-purpose hametz to realize the industrialization big production.Through produces the sour speed research to two fungi to discover that PP, LP produce the sour ability to be quick, two fungus's fermentation supernate bacteriostasis effects are obvious, the mixed culture experiment suppresses the putrefactive bacteria effect to be remarkable. The plant lactobacillus and the pentose piece coccus is suitable the growth temperature respectively be 37℃and 30℃, this kind is suitable the growth temperature difference to be advantageous in displays two kind of lactobacilli to achieve the manufacture high quality fermented feed in the feed different fermentation stage supplementary function the production goal; Two strains comparatively speaking, the plant lactobacillus's biomass must be slightly high a spot, but pentose piece coccus's effective colony quantity wants obvious high 5%. The ventilation or not and the vaccination quantity size is not remarkable regarding the mycelium biomass influence. The vaccination quantity's influence in the logarithmic growth initial period is obvious, enters the logarithmic growth last stage influence not to be remarkable, regarding the biggest biomass which achieves respectively also does not have the remarkable influence.The research uses the sea alginic acid sodium - gelatin to take the pouch material, the solidification fluid uses the calcium chloride solution, the experimental effect proved that this method suits in preserved plant lactobacillus LP, enhanced the lactobacillus to the dry resistance. Formulates finally the freezing and drying craft was according to the fast temperature decrease, the slow elevation of temperature principle, the heat preservation process time length had mainly considered the freezing and drying remaining moisture content's influence, kept warm the 360min remaining moisture content to be in 3~5% basically, the experimental result demonstrated within this scope could obtain the high live fungus survival percentage. The freezing and drying protecting agent's research demonstration protecting agent's type is inconsistent to PP and the LP fungus's protection effect, the glycerine, the phaseomannite and Vitamin C are strongest to the PP fungus's protective function, but to the LP fungus's freezing and drying protection experiment is Vitamin C, the glycerine and the powdered sugar protective function is strongest. In the protecting agent Vitamin C and the glycerine display the strong protective function.Changes of total plate count, lactic acid bacteria, coliform bacteria in fermentation feed were investigated in this paper. The results showed that lactic acid bacteria amount evidently increased and had a maximum value and 2.5% of lactic acid content after 48 hours'fermentation, as well as coliform bacteria were inhabited in feed. This study was designed to determine the effect of dietary for weaned piglets. sixty piglets weaned at 32 days of age were randomly assigned to 3 treatment groups, representing soybean meal-based diets, or fermentation feed, fermentation feed(50%)and soybean meal-based diets(50%). The results show: Compared with the control group, the Average-day-gain and voluntary feed intake of experimental group I and II increased by 8.61%,5.06% and 4.08%,7.59%.
Keywords/Search Tags:fermentation feed, lactobacillus, optimum culture conditions, freeze-drying technology
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